The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

Table of contents

About

Terms

 

Gene Review: 

BMEII0032  -  VirB8

Brucella melitensis bv. 1 str. 16M

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of BMEII0032

  • Structural comparison of pVirB8(AT) with Brucella suis VirB8 confirms that the monomers have a similar fold [1].
  • Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites [1].
  • Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori [2].
  • Here we present crystal structures of VirB8 of Brucella suis, the causative agent of brucellosis, and ComB10, a VirB10 homolog of Helicobacter pylori, the causative agent of gastric ulcers [2].
  • Genes encoding VirB1, VirB8, VirB9, VirB10 and VirB11 were cloned into expression vectors; the affinity-tagged proteins were purified from Escherichia coli, and analyses by gel filtration chromatography showed that they form monomers or homo-multimers [3].
 

High impact information on BMEII0032

  • Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity [4].
  • The in vivo functionality of VirB8 variants was determined by complementation of growth in macrophages by a B. suis virB8 mutant and by using a heterologous assay of type IV secretion system assembly in Agrobacterium tumefaciens [4].
  • VirB8-like proteins are essential components of type IV secretion systems, bacterial virulence factors that mediate the translocation of effector molecules from many bacterial pathogens into eukaryotic cells [4].
  • Complementation with the B. suis VirB8 protein expressed from the virB promoter restored virulence [5].
  • To analyze the production of VirB proteins, polyclonal antisera against B. suis VirB5 and VirB8 were generated [6].
 

Analytical, diagnostic and therapeutic context of BMEII0032

  • Western blot analysis revealed that VirB5 and VirB8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation [6].

References

  1. Agrobacterium tumefaciens VirB8 structure reveals potential protein-protein interaction sites. Bailey, S., Ward, D., Middleton, R., Grossmann, J.G., Zambryski, P.C. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  2. Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori. Terradot, L., Bayliss, R., Oomen, C., Leonard, G.A., Baron, C., Waksman, G. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  3. The putative lytic transglycosylase VirB1 from Brucella suis interacts with the type IV secretion system core components VirB8, VirB9 and VirB11. Höppner, C., Carle, A., Sivanesan, D., Hoeppner, S., Baron, C. Microbiology (Reading, Engl.) (2005) [Pubmed]
  4. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Paschos, A., Patey, G., Sivanesan, D., Gao, C., Bayliss, R., Waksman, G., O'callaghan, D., Baron, C. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  5. Swapping of periplasmic domains between Brucella suis VirB8 and a pSB102 VirB8 homologue allows heterologous complementation. Patey, G., Qi, Z., Bourg, G., Baron, C., O'Callaghan, D. Infect. Immun. (2006) [Pubmed]
  6. Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera. Rouot, B., Alvarez-Martinez, M.T., Marius, C., Menanteau, P., Guilloteau, L., Boigegrain, R.A., Zumbihl, R., O'Callaghan, D., Domke, N., Baron, C. Infect. Immun. (2003) [Pubmed]
Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities