High impact information on Bop1

• Pes1 mutants defective for the interaction with Bop1 lose the ability to affect rRNA maturation and the cell cycle [1].
• Physical and functional interaction between Pes1 and Bop1 in mammalian ribosome biogenesis [1].
• Bop1 is a novel nucleolar protein involved in rRNA processing and ribosome assembly [2].
• Perturbation of Bop1 activities by Bop1Delta also induces a powerful yet reversible cell cycle arrest in 3T3 fibroblasts [2].
• RNase A treatment disrupts these particles and releases Bop1 into a low-molecular-weight fraction [3].

Biological context of Bop1

• Sequence analysis of the 5'-untranslated region of HSF-1 suggests that it contains sequences of a recently described Bop1 gene in reverse orientation within its first 331 base pairs (bp) upstream of the translation initiation site [4].
• ERB1, the yeast homolog of mammalian Bop1, is an essential gene required for maturation of the 25S and 5.8S ribosomal RNAs [5].
• Here we have investigated the functions of the Saccharomyces cerevisiae homolog of Bop1, Erb1p, encoded by the previously uncharacterized open reading frame YMR049C [5].

Anatomical context of Bop1

• We have identified and characterized a novel mouse protein, Bop1, which contains WD40 repeats and is highly conserved through evolution. bop1 is ubiquitously expressed in all mouse tissues examined and is upregulated during mid-G(1) in serum-stimulated fibroblasts [3].

Associations of Bop1 with chemical compounds

• In sucrose density gradients, Bop1 from nuclear extracts cosediments with the 50S-80S ribonucleoprotein particles that contain the 32S rRNA precursor [3].

Other interactions of Bop1

• Bop1 is a conserved nucleolar protein involved in rRNA processing and ribosome assembly in eukaryotes [6].

Analytical, diagnostic and therapeutic context of Bop1

• Immunofluorescence analysis shows that Bop1 is localized predominantly to the nucleolus [3].

References