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Gene Review:

pTi_009 - gene 4' protein

Agrobacterium tumefaciens

Dehio, C. et al., Sibold, L. et al., Yusibov, V.M. et al.

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Disease relevance of pTi_009

The SrEnod2 gene was also found to be highly expressed in S. rostrata crown gall tumors induced by wild-type Agrobacterium tumefaciens strains, but not in tumors induced by an A. tumefaciens strain carrying a mutation in the cytokinin biosynthesis gene 4 [1].
A plasmid was constructed that directs expression of the TL-DNA gene 4 protein in E. coli [2].

High impact information on pTi_009

The tumour-inducing T-DNA gene 4 (T-cyt gene) of the nopaline Ti plasmid pTiC58 was cloned and introduced into tobacco cells by leaf disc transformation using Agrobacterium plasmid vectors [3].
DNA sequencing analysis showed that pGV793 carried a hybrid ribosome binding site composed of the cro SD sequence, a five bp sequence and the ATG codon of gene 4 [2].

References

The early nodulin gene SrEnod2 from Sesbania rostrata is inducible by cytokinin. Dehio, C., de Bruijn, F.J. Plant J. (1992) [Pubmed]
Cloning and expression in Escherichia coli of the TL-DNA gene 4 of Agrobacterium tumefaciens under the control of the PR promoter of bacteriophage lambda. Sibold, L., Guiso, N., de Beuckeleer, M., Van Montagu, M. Biochimie (1984) [Pubmed]
Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant gene expression in response to phytohormonal stress. Yusibov, V.M., Il, P.C., Andrianov, V.M., Piruzian, E.S. Plant Mol. Biol. (1991) [Pubmed]

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