Disease relevance of bioS

• Sinorhizobium meliloti 1021 responds to external biotin signals from alfalfa plants through the bioS regulatory locus [1].
• A novel biotin-inducible DNA locus, bioS, was found in Rhizobium meliloti 1021 by mutation with a transposable promoter probe [2].
• Expression of bioS in Escherichia coli resulted in the production of a 35-kDa BioS protein with characteristics of an LysR-type regulator [3].

High impact information on bioS

• Mutation of bioS increases biotin uptake, extends stationary phase in the presence of biotin, and impairs competitive growth in the presence of biotin [3].
• The absence in BioS of any significant homology to known amino acid sequences was consistent with the fact that DNA hybridization tests with seven genera of bacteria detected bioS only in Sinorhizobium [3].
• The 588-bp bioS is located among three genes showing homology to survival operons of other bacteria, and it may be part of a system that R. meliloti uses to respond to plant biotin signals [2].
• Comparison of the bdhA and the bioS promoter regions identified several common motifs [1].

Biological context of bioS

• Those traits included a deduced amino acid sequence containing possible helix-turn-helix DNA-binding structures and three T-N(11)-A motifs upstream from the bioS gene [3].

References