Disease relevance of yscM1

• However, overproduction of YscM from a multicopy plasmid in wild-type Yersinia enterocolitica prevented Yop secretion and synthesis [1].
• Yersinia enterocolitica transports YscM1 and YscM2 via the type III pathway, a mechanism that is required for the establishment of bacterial infections [2].
• We have recombinantly expressed and purified YscM1 and YscM2 in Escherichia coli [3].

High impact information on yscM1

• YscM1, the transciptional regulator of Yersinia enterocolitica, is also injected into eukaryotic cells, whereas the related YscM2 protein remains associated with bacterial cells [4].
• However, no effect could be observed in the absence of a pYV plasmid, indicating that YscM1 does not act directly as a transcriptional repressor or as an anti-VirF factor [5].
• Prior to host cell contact, YscM1 and YscM2 exert posttranscriptional regulation to inhibit expression of effector yop genes, which encode virulence factors that travel the type III pathway into the cytoplasm of macrophages [2].

Biological context of yscM1

• YscM1 and YscM2, two Yersinia enterocolitica proteins causing downregulation of yop transcription [5].
• The YscM1 and YscM2 homodimers could be dissociated at high ionic strength, indicating that salt bridges essentially contribute to the dimerization [3].

Associations of yscM1 with chemical compounds

• Expression of C-terminal fusions of YscM1 and YscM2 to the neomycin phosphotransferase reporter revealed sequences required for regulatory activity and for secretion in the absence of SycH [2].

Analytical, diagnostic and therapeutic context of yscM1

• Gel filtration analyses revealed that both YscM1 and YscM2 formed homodimers [3].

References