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Gene Review:

prpE - propionate--CoA ligase

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Valentin, H.E. et al., Horswill, A.R. et al.

Valentin, Mitsky, Mahadeo, Tran, Gruys,

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Disease relevance of prpE

The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase [1].
When prpE was coexpressed with the polyhydroxyalkanoate (PHA) biosynthetic genes from Ralstonia eutropha in recombinant E. coli, a PHA copolymer containing 3HP units accumulated when 3HP was supplied with the growth medium [2].

High impact information on prpE

The genetic operon for propionic acid degradation in Salmonella enterica serovar Typhimurium contains an open reading frame designated prpE which encodes a propionyl coenzyme A (propionyl-CoA) synthetase (A. R. Horswill and J. C. Escalante-Semerena, Microbiology 145:1381-1388, 1999) [2].
In this paper we report the cloning of prpE by PCR, its overexpression in Escherichia coli, and the substrate specificity of the enzyme [2].
Biochemical and genetic evidence is presented to demonstrate that the prpE gene of Salmonella typhimurium encodes propionyl-CoA synthetase, an enzyme required for the catabolism of propionate in this bacterium [1].

References

The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase. Horswill, A.R., Escalante-Semerena, J.C. Microbiology (Reading, Engl.) (1999) [Pubmed]
Application of a propionyl coenzyme A synthetase for poly(3-hydroxypropionate-co-3-hydroxybutyrate) accumulation in recombinant Escherichia coli. Valentin, H.E., Mitsky, T.A., Mahadeo, D.A., Tran, M., Gruys, K.J. Appl. Environ. Microbiol. (2000) [Pubmed]

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