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Gene Review:

melB - melibiose:sodium symporter

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Shimamoto, T. et al., Kuroda, M. et al., Sanderson, K.E. et al., Mizushima, K. et al.

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Disease relevance of melB

Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2 [1].

High impact information on melB

We cloned the mutated melB gene that encodes the melibiose carrier, determined the nucleotide sequences, and identified replaced nucleotides [2].
The locations of IS200-I, IS200-III, and IS200-V were confirmed to be close to sufD, melB, and purC, as previously indicated [3].
Although the cloned melB gene is closely similar to the melB genes of other bacteria, it is cryptic because of a frameshift mutation [4].

Analytical, diagnostic and therapeutic context of melB

Site-directed mutagenesis was used to construct a functional melB gene by deleting one nucleotide, resulting in the production of an active melibiose transporter [4].

References

Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2. Mizushima, K., Awakihara, S., Kuroda, M., Ishikawa, T., Tsuda, M., Tsuchiya, T. Mol. Gen. Genet. (1992) [Pubmed]
Resistance of the melibiose carrier to inhibition by the phosphotransferase system due to substitutions of amino acid residues in the carrier of Salmonella typhimurium. Kuroda, M., de Waard, S., Mizushima, K., Tsuda, M., Postma, P., Tsuchiya, T. J. Biol. Chem. (1992) [Pubmed]
Location of IS200 on the genomic cleavage map of Salmonella typhimurium LT2. Sanderson, K.E., Sciore, P., Liu, S.L., Hessel, A. J. Bacteriol. (1993) [Pubmed]
A cryptic melibiose transporter gene possessing a frameshift from Citrobacter freundii. Shimamoto, T., Shimamoto, T., Xu, X.J., Okazaki, N., Kawakami, H., Tsuchiya, T. J. Biochem. (2001) [Pubmed]

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