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Gene Review

miaE  -  tRNA-(ms[2]io[6]A)-hydroxylase

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

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Disease relevance of miaE


High impact information on miaE


Biological context of miaE

  • Mutants of S. typhimurium which have MudJ inserted in the miaE gene and which, consequently, are blocked in the ms2i6A hydroxylation reaction were isolated [1].
  • Its DNA sequence and transcription pattern together with complementation studies revealed that the miaE gene is the second gene of a dicistronic operon [1].
  • The most pronounced alterations consisted in a 2.6-fold induction of aryl hydrocarbon hydroxylase in the lung and 8-fold induction of ethoxyresorufin deethylase in the liver, and in a marked stimulation of the liver metabolic activation of all promutagens [6].
  • However, any significant correlation between aniline hydroxylase activity and the mutagenesis was not observed [7].

Anatomical context of miaE

  • AHH activity and total B[a]P hydroxylase activity were also decreased in these liver microsomes [8].
  • The monitored biochemical parameters included aryl hydrocarbon (benzo[a]pyrene) hydroxylase and ethoxyresorufin deethylase in microsomal fractions, epoxide (benzo[a]pyrene-4,5-oxide) hydrolase in both microsomal and cytosolic fractions, glutathione (GSH) and GSH S-transferase in the cytosol [6].

Associations of miaE with chemical compounds


Analytical, diagnostic and therapeutic context of miaE


  1. Isolation of the gene (miaE) encoding the hydroxylase involved in the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella typhimurium and characterization of mutants. Persson, B.C., Björk, G.R. J. Bacteriol. (1993) [Pubmed]
  2. Quinoline: conversion to a mutagen by human and rodent liver. Hollstein, M., Talcott, R., Wei, E. J. Natl. Cancer Inst. (1978) [Pubmed]
  3. Androgen-dependent renal microsomal cytochrome P-450 responsible for N-hydroxylation and mutagenic activation of 3-methoxy-4-aminoazobenzene in the BALB/c mouse. Degawa, M., Miura, S., Hashimoto, Y. Cancer Res. (1990) [Pubmed]
  4. Reduction of chromium(VI) to chromium(V) by rat liver cytosolic and microsomal fractions: is DT-diaphorase involved? Aiyar, J., De Flora, S., Wetterhahn, K.E. Carcinogenesis (1992) [Pubmed]
  5. The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates. Persson, B.C., Olafsson, O., Lundgren, H.K., Hederstedt, L., Björk, G.R. J. Bacteriol. (1998) [Pubmed]
  6. Metabolic alterations produced by cigarette smoke in rat lung and liver, and their modulation by oral N-acetylcysteine. Bagnasco, M., Bennicelli, C., Camoirano, A., Balansky, R.M., De Flora, S. Mutagenesis (1992) [Pubmed]
  7. Differential mutagenicities of triamino benzenes against Salmonella typhimurium TA98 in the presence of S9 fractions from polychlorinated biphenyls-, phenobarbital- or 3-methylcholanthrene-pretreated rats, hamsters and mice. Yoshikawa, K., Nohmi, T., Harada, R., Inokawa, Y., Ishidate, M. The Journal of toxicological sciences. (1979) [Pubmed]
  8. Suppressed mutagenicity of benzo[a]pyrene by the liver S9 fraction and microsomes from eugenol-treated rats. Yokota, H., Hoshino, J., Yuasa, A. Mutat. Res. (1986) [Pubmed]
  9. Antimutagenic and mutagenic potentials of Chinese radish. Rojanapo, W., Tepsuwan, A. Environ. Health Perspect. (1993) [Pubmed]
  10. Comparison of rat and guinea pig as sources of the S9 fraction in the Salmonella/mammalian microsome mutagenicity test. Baker, R.S., Bonin, A.M., Stupans, I., Holder, G.M. Mutat. Res. (1980) [Pubmed]
  11. In vitro effects of L-ascorbic acid (vitamin C) on aryl hydrocarbon hydroxylase activity in hepatic microsomes of mice. Kiyohara, C., Omura, M., Hirohata, T. Mutat. Res. (1991) [Pubmed]
  12. Evidence for the activation of 3-methylcholanthrene as a carcinogen in vivo and asa mutagen in vitro by P1 -450 from inbred strains of mice. Nebert, D.W., Felton, J.S. Adv. Exp. Med. Biol. (1975) [Pubmed]
  13. Metabolizing activities of postmitochondrial liver fractions (S-9) on benzo[a]pyrene and dimethylnitrosamine. Teichmann, B., Butschak, G., Scheunig, G., Köhler, S. Arch. Toxicol. Suppl. (1980) [Pubmed]
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