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Gene Review

denV  -  bifunctional N-glycosylase and DNA...

Enterobacteria phage T4

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Disease relevance of denV

  • Identification, physical map location and sequence of the denV gene from bacteriophage T4 [1].
  • Increased resistance to u.v. light and more rapid recovery of replicative DNA synthesis following u.v. irradiation have been correlated both with improved repair DNA synthesis and with a novel dimer incision capability present in denV transfected Xeroderma cells but not as evident in transfected normal cells [2].
  • Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli [3].
  • The ability of the denV gene to restore the DNA repair deficiency in XP fibroblasts was examined using host cell reactivation of viral structural antigen formation for UV-irradiated adenovirus [4].
  • In this work we have constructed a helper-independent recombinant human adenovirus, Ad5denV, which contains the denV gene [4].

High impact information on denV


Chemical compound and disease context of denV


Biological context of denV

  • The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene [1].
  • The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078 [1].
  • This mutant strain rescued the denV+ allele from plasmids containing the wild-type sequence [10].
  • Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4 [10].
  • Confirmation that this open reading frame is part of the denV coding sequence was obtained by identifying a TAG amber codon in the homologous DNA derived from a denV amber mutant strain [10].

Anatomical context of denV


Associations of denV with chemical compounds

  • The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV [3].
  • The denV transfected clones and liposome-treated cells showed increased unscheduled DNA synthesis and enhanced removal of pyrimidine dimers compared to controls [9].
  • In addition, the recombination defective mutants xm(uvsX) and y10(y) were sensitive to MNNG than wild-type, whereas mutants defective in genes 45 and denV, which are apparently not involved in recombination, were not MNNG sensitive [12].
  • When removal of UVR-induced photoproducts was initiated by endogenous excision repair mechanisms, an average of 38 nucleotides were replaced per dimer removed, as determined by bromouracil photolysis; denV-initiated excision repair, on the other hand, resulted in removal of an average of 6 nucleotides per dimer repaired [13].
  • These results demonstrate the feasibility of using the cloned denV gene with its well characterized pyrimidine cyclobutane dimer-specific endonuclease activity to reconstitute UV-induced DNA repair in human cells deficient in DNA repair [14].

Other interactions of denV

  • Requirements for the dispensable replication and recombination genes of T4 were probed; T4 topoisomerase (39, 52, 60), primase (58/61), and uvsX are required, whereas the host recA gene and T4 denV gene do not appear to be required for isolation of the Hp17 mutants [15].

Analytical, diagnostic and therapeutic context of denV


  1. Identification, physical map location and sequence of the denV gene from bacteriophage T4. Valerie, K., Henderson, E.E., deRiel, J.K. Nucleic Acids Res. (1984) [Pubmed]
  2. Restoration of u.v.-induced excision repair in Xeroderma D cells transfected with the denV gene of bacteriophage T4. Arrand, J.E., Squires, S., Bone, N.M., Johnson, R.T. EMBO J. (1987) [Pubmed]
  3. Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli. Lloyd, R.S., Hanawalt, P.C. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  4. Construction of a recombinant adenovirus containing the denV gene from bacteriophage T4 which can partially restore the DNA repair deficiency in xeroderma pigmentosum fibroblasts. Colicos, M.A., Haj-Ahmad, Y., Valerie, K., Henderson, E.E., Rainbow, A.J. Carcinogenesis (1991) [Pubmed]
  5. denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster. Banga, S.S., Boyd, J.B., Valerie, K., Harris, P.V., Kurz, E.M., de Riel, J.K. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  6. Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4. Valerie, K., de Riel, J.K., Henderson, E.E. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  7. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA. Nakabeppu, Y., Sekiguchi, M. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  8. Photodynamic inactivation and mutagenesis by angelicin (isopsoralen) or thiopyronin (methylene red) in wild-type and repair-deficient strains of bacteriophage T4. Drake, J.W. J. Bacteriol. (1985) [Pubmed]
  9. Enhancement of ultraviolet-DNA repair in denV gene transfectants and T4 endonuclease V-liposome recipients. Kibitel, J.T., Yee, V., Yarosh, D.B. Photochem. Photobiol. (1991) [Pubmed]
  10. Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4. Radany, E.H., Naumovski, L., Love, J.D., Gutekunst, K.A., Hall, D.H., Friedberg, E.C. J. Virol. (1984) [Pubmed]
  11. Enhanced pyrimidine dimer repair in cultured murine epithelial cells transfected with the denV gene of bacteriophage T4. Kusewitt, D.F., Budge, C.L., Ley, R.D. J. Invest. Dermatol. (1994) [Pubmed]
  12. Recombinational repair of alkylation lesions in phage T4. I. N-methyl-N'-nitro-N-nitrosoguanidine. Schneider, S., Bernstein, C., Bernstein, H. Mol. Gen. Genet. (1978) [Pubmed]
  13. Enhanced pyrimidine dimer removal in repair-proficient murine fibroblasts transformed with the denV gene of bacteriophage T4. Kusewitt, D.F., Ley, R.D., Henderson, E.E. Mutat. Res. (1991) [Pubmed]
  14. Host cell reactivation of CAT-expression vectors as a method to assay for cloned DNA-repair genes. Henderson, E.E., Valerie, K., Green, A.P., de Riel, J.K. Mutat. Res. (1989) [Pubmed]
  15. Reiterated gene amplifications at specific short homology sequences in phage T4 produce Hp17 mutants. Wu, D.G., Wu, C.H., Black, L.W. J. Mol. Biol. (1991) [Pubmed]
  16. Microinjection of T4 endonuclease V produced by a synthetic denV gene stimulates unscheduled DNA synthesis in both xeroderma pigmentosum and normal cells. Yamaizumi, M., Inaoka, T., Uchida, T., Ohtsuka, E. Mutat. Res. (1989) [Pubmed]
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