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Gene Review

denV  -  bifunctional N-glycosylase and DNA...

Enterobacteria phage T4

 
 
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Disease relevance of denV

  • Identification, physical map location and sequence of the denV gene from bacteriophage T4 [1].
  • Increased resistance to u.v. light and more rapid recovery of replicative DNA synthesis following u.v. irradiation have been correlated both with improved repair DNA synthesis and with a novel dimer incision capability present in denV transfected Xeroderma cells but not as evident in transfected normal cells [2].
  • Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli [3].
  • The ability of the denV gene to restore the DNA repair deficiency in XP fibroblasts was examined using host cell reactivation of viral structural antigen formation for UV-irradiated adenovirus [4].
  • In this work we have constructed a helper-independent recombinant human adenovirus, Ad5denV, which contains the denV gene [4].
 

High impact information on denV

 

Chemical compound and disease context of denV

 

Biological context of denV

  • The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene [1].
  • The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078 [1].
  • This mutant strain rescued the denV+ allele from plasmids containing the wild-type sequence [10].
  • Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4 [10].
  • Confirmation that this open reading frame is part of the denV coding sequence was obtained by identifying a TAG amber codon in the homologous DNA derived from a denV amber mutant strain [10].
 

Anatomical context of denV

 

Associations of denV with chemical compounds

  • The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV [3].
  • The denV transfected clones and liposome-treated cells showed increased unscheduled DNA synthesis and enhanced removal of pyrimidine dimers compared to controls [9].
  • In addition, the recombination defective mutants xm(uvsX) and y10(y) were sensitive to MNNG than wild-type, whereas mutants defective in genes 45 and denV, which are apparently not involved in recombination, were not MNNG sensitive [12].
  • When removal of UVR-induced photoproducts was initiated by endogenous excision repair mechanisms, an average of 38 nucleotides were replaced per dimer removed, as determined by bromouracil photolysis; denV-initiated excision repair, on the other hand, resulted in removal of an average of 6 nucleotides per dimer repaired [13].
  • These results demonstrate the feasibility of using the cloned denV gene with its well characterized pyrimidine cyclobutane dimer-specific endonuclease activity to reconstitute UV-induced DNA repair in human cells deficient in DNA repair [14].
 

Other interactions of denV

  • Requirements for the dispensable replication and recombination genes of T4 were probed; T4 topoisomerase (39, 52, 60), primase (58/61), and uvsX are required, whereas the host recA gene and T4 denV gene do not appear to be required for isolation of the Hp17 mutants [15].
 

Analytical, diagnostic and therapeutic context of denV

References

  1. Identification, physical map location and sequence of the denV gene from bacteriophage T4. Valerie, K., Henderson, E.E., deRiel, J.K. Nucleic Acids Res. (1984) [Pubmed]
  2. Restoration of u.v.-induced excision repair in Xeroderma D cells transfected with the denV gene of bacteriophage T4. Arrand, J.E., Squires, S., Bone, N.M., Johnson, R.T. EMBO J. (1987) [Pubmed]
  3. Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli. Lloyd, R.S., Hanawalt, P.C. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  4. Construction of a recombinant adenovirus containing the denV gene from bacteriophage T4 which can partially restore the DNA repair deficiency in xeroderma pigmentosum fibroblasts. Colicos, M.A., Haj-Ahmad, Y., Valerie, K., Henderson, E.E., Rainbow, A.J. Carcinogenesis (1991) [Pubmed]
  5. denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster. Banga, S.S., Boyd, J.B., Valerie, K., Harris, P.V., Kurz, E.M., de Riel, J.K. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  6. Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4. Valerie, K., de Riel, J.K., Henderson, E.E. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  7. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA. Nakabeppu, Y., Sekiguchi, M. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  8. Photodynamic inactivation and mutagenesis by angelicin (isopsoralen) or thiopyronin (methylene red) in wild-type and repair-deficient strains of bacteriophage T4. Drake, J.W. J. Bacteriol. (1985) [Pubmed]
  9. Enhancement of ultraviolet-DNA repair in denV gene transfectants and T4 endonuclease V-liposome recipients. Kibitel, J.T., Yee, V., Yarosh, D.B. Photochem. Photobiol. (1991) [Pubmed]
  10. Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4. Radany, E.H., Naumovski, L., Love, J.D., Gutekunst, K.A., Hall, D.H., Friedberg, E.C. J. Virol. (1984) [Pubmed]
  11. Enhanced pyrimidine dimer repair in cultured murine epithelial cells transfected with the denV gene of bacteriophage T4. Kusewitt, D.F., Budge, C.L., Ley, R.D. J. Invest. Dermatol. (1994) [Pubmed]
  12. Recombinational repair of alkylation lesions in phage T4. I. N-methyl-N'-nitro-N-nitrosoguanidine. Schneider, S., Bernstein, C., Bernstein, H. Mol. Gen. Genet. (1978) [Pubmed]
  13. Enhanced pyrimidine dimer removal in repair-proficient murine fibroblasts transformed with the denV gene of bacteriophage T4. Kusewitt, D.F., Ley, R.D., Henderson, E.E. Mutat. Res. (1991) [Pubmed]
  14. Host cell reactivation of CAT-expression vectors as a method to assay for cloned DNA-repair genes. Henderson, E.E., Valerie, K., Green, A.P., de Riel, J.K. Mutat. Res. (1989) [Pubmed]
  15. Reiterated gene amplifications at specific short homology sequences in phage T4 produce Hp17 mutants. Wu, D.G., Wu, C.H., Black, L.W. J. Mol. Biol. (1991) [Pubmed]
  16. Microinjection of T4 endonuclease V produced by a synthetic denV gene stimulates unscheduled DNA synthesis in both xeroderma pigmentosum and normal cells. Yamaizumi, M., Inaoka, T., Uchida, T., Ohtsuka, E. Mutat. Res. (1989) [Pubmed]
 
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