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Ddx5  -  DEAD (Asp-Glu-Ala-Asp) box polypeptide 5

Mus musculus

Synonyms: 2600009A06Rik, DEAD box RNA helicase DEAD1, DEAD box protein 5, G17P1, HUMP68, ...
 
 
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High impact information on Ddx5

  • The immunodominant antigen of an ultraviolet-induced regressor tumor is generated by a somatic point mutation in the DEAD box helicase p68 [1].
  • The peptide, SNFVFAGI, matched the sequence of the DEAD box protein p68 RNA helicase except for a single amino acid substitution, caused by a single nucleotide change [1].
  • These monoclonal antibodies (class IgG1) can identify the electrophoresed protein (p68) in polyacrylamide gels by the electrophoretic transfer blotting technique [2].
  • Such immune complex preparations have associated protein kinase activity--i.e., addition of [gamma-32P]ATP results in the phosphorylation of p68 and added substrates, calf thymus histone, and eukaryotic initiation factor 2 [2].
  • Mutations in the putative zinc fingers present in the carboxyl terminus of p180 abolished the interaction with p68 completely, although the mutants were still capable of interacting with p54-p46 [3].
 

Biological context of Ddx5

 

Anatomical context of Ddx5

  • The p68 substrate was also phosphorylated in a cell-free system under conditions designed to activate PK-C [5].
  • We propose that phosphorylation of the p68 molecule is a physiochemical marker for the activation of PK-C in myeloid cells, in response to the growth-promoting physiological ligand [5].
  • Homogenates and plasma membrane fractions from clones highly responsive to ACTH had large amounts of p68 [7].
  • 3T3 cells containing a nontransforming oncoprotein p26-BCL2, in contrast, exhibited normal levels and distribution of p68-MARCKS [9].
  • The biological effects of interleukin-1 (IL-1) are mediated by two distinct receptors, the p80 type I IL-1 and p68 type II IL-1 receptor proteins (IL-1RI and IL-1RII, respectively), both of which have been recently co-localized to the growth hormone synthesizing cells of the adenohypophysis [10].
 

Associations of Ddx5 with chemical compounds

  • Phosphoamino acid analysis demonstrated that the p68 molecule phosphorylated in intact cells as well as in a calcium-phospho-lipid-dependent cell-free system was phosphorylated on threonine residues, not tyrosine [5].
  • Phorbol ester and ionomycin treatment induced p50, p65, and p68 c-rel kappa B DNA-binding activity [6].
 

Analytical, diagnostic and therapeutic context of Ddx5

References

  1. The immunodominant antigen of an ultraviolet-induced regressor tumor is generated by a somatic point mutation in the DEAD box helicase p68. Dubey, P., Hendrickson, R.C., Meredith, S.C., Siegel, C.T., Shabanowitz, J., Skipper, J.C., Engelhard, V.H., Hunt, D.F., Schreiber, H. J. Exp. Med. (1997) [Pubmed]
  2. Monoclonal antibodies to an interferon-induced Mr 68,000 protein and their use for the detection of double-stranded RNA-dependent protein kinase in human cells. Laurent, A.G., Krust, B., Galabru, J., Svab, J., Hovanessian, A.G. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  3. Molecular architecture of the mouse DNA polymerase alpha-primase complex. Mizuno, T., Yamagishi, K., Miyazawa, H., Hanaoka, F. Mol. Cell. Biol. (1999) [Pubmed]
  4. Assignment to chromosome 11 of mouse p68 RNA helicase gene (Hlr1) and pseudogene (Hlr1-ps1). Petry, P., Johnson, K.R., Lemaire, L., Heinlein, U.A. FEBS Lett. (1995) [Pubmed]
  5. Multilineage hematopoietic growth factor interleukin 3 and direct activators of protein kinase C stimulate phosphorylation of common substrates. Evans, S.W., Rennick, D., Farrar, W.L. Blood (1986) [Pubmed]
  6. Expression and induction of nuclear factor-kappa B-related proteins in thymocytes. Sen, J., Venkataraman, L., Shinkai, Y., Pierce, J.W., Alt, F.W., Burakoff, S.J., Sen, R. J. Immunol. (1995) [Pubmed]
  7. Association of a 68,000-dalton protein with adrenocorticotropin-sensitive adenylate cyclase activity in Y1 adrenocortical tumor cells. Watt, V.M., Schimmer, B.P. J. Biol. Chem. (1981) [Pubmed]
  8. Cloning and characterization of the 5'-upstream sequence governing the cell cycle-dependent transcription of mouse DNA polymerase alpha 68 kDa subunit gene. Nishikawa, N.S., Izumi, M., Uchida, H., Yokoi, M., Miyazawa, H., Hanaoka, F. Nucleic Acids Res. (2000) [Pubmed]
  9. Transformed 3T3 cells have reduced levels and altered subcellular distribution of the major PKC substrate protein MARCKS. Reed, J.C., Rapp, U., Cuddy, M.P. Cell. Signal. (1991) [Pubmed]
  10. Expression and localization of p80 and p68 interleukin-1 receptor proteins in the brain of adult mice. French, R.A., VanHoy, R.W., Chizzonite, R., Zachary, J.F., Dantzer, R., Parnet, P., Bluthé, R.M., Kelley, K.W. J. Neuroimmunol. (1999) [Pubmed]
 
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