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Gene Review

arsD  -  ArsD

Escherichia coli

 
 
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Disease relevance of arsD

  • In this article, we report identification of an arsenic chaperone, ArsD, encoded by the arsRDABC operon of Escherichia coli [1].
 

High impact information on arsD

  • Interaction with ArsD increases the affinity of ArsA for arsenite, thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion [1].
  • ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)/Sb(III) efflux pump [1].
  • Disruption of the Cys(112)-Cys(113) site by mutagenesis of arsD, but not the Cys(12)-Cys(13) site, largely abolished this cooperativity, indicative of interactions between adjacent Cys(112)-Cys(113) sites within the dimer [2].
  • We propose a model involving the sequential binding and release of metalloids by the four binding sites of dimeric ArsD, with only one site releasing free metalloids [2].
  • ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli [2].
 

Chemical compound and disease context of arsD

 

Biological context of arsD

 

Associations of arsD with chemical compounds

  • It possesses two-pairs of vicinal cysteine residues, Cys(12)-Cys(13) and Cys(112)-Cys(113), that potentially form separate binding sites for the metalloids that trigger dissociation of ArsD from the operon [2].
 

Analytical, diagnostic and therapeutic context of arsD

  • Titration of the steady-state fluorescence of ArsD with metalloids revealed positive cooperativity, with a Hill coefficient of 2, between these sites [2].
  • Western blot analysis identified the R46 proteins using antibodies against R773 ArsA, ArsD and ArsR [5].

References

  1. An arsenic metallochaperone for an arsenic detoxification pump. Lin, Y.F., Walmsley, A.R., Rosen, B.P. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  2. Evidence for cooperativity between the four binding sites of dimeric ArsD, an As(III)-responsive transcriptional regulator. Li, S., Rosen, B.P., Borges-Walmsley, M.I., Walmsley, A.R. J. Biol. Chem. (2002) [Pubmed]
  3. Role of vicinal cysteine pairs in metalloid sensing by the ArsD As(III)-responsive repressor. Li, S., Chen, Y., Rosen, B.P. Mol. Microbiol. (2001) [Pubmed]
  4. The arsD gene encodes a second trans-acting regulatory protein of the plasmid-encoded arsenical resistance operon. Wu, J., Rosen, B.P. Mol. Microbiol. (1993) [Pubmed]
  5. The arsenical resistance operon of IncN plasmid R46. Bruhn, D.F., Li, J., Silver, S., Roberto, F., Rosen, B.P. FEMS Microbiol. Lett. (1996) [Pubmed]
 
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