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Gene Review:

PRRSVgp7 - membrane protein M

Porcine reproductive and respiratory syndrome virus

Dobbe, J.C. et al., Kreutz, L.C. et al., van Nieuwstadt, A.P. et al., Meulenberg, J.J. et al., Meulenberg, J.J. et al.

Kreutz, Mengeling, Meulenberg, Petersen-den Besten, Meulenberg,

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Disease relevance of PRRSVgp7

Previously we have shown that Lelystad virus (LV) contains five structural proteins, a nucleocapsid protein N, an integral membrane protein M, and three glycoproteins GP3, GP4, and GP5 [1].
Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycoprotein GP(5) and the membrane protein M, were replaced by sequences from envelope proteins of related and unrelated RNA viruses [2].

High impact information on PRRSVgp7

In addition to the 18-kDa integral membrane protein M and the 15-kDa nucleocapsid protein N, two new structural proteins with molecular masses of 45 to 50 kDa and 31 to 35 kDa, respectively, were detected [3].
Each of the three major structural proteins (envelope glycoprotein E, nonglycosylated membrane protein M, and nucleoprotein N) of an American strain of porcine reproductive and respiratory syndrome virus (PRRSV) was expressed using a recombinant baculovirus expression system [4].

Biological context of PRRSVgp7

The genome encodes the RNA replicase (ORF1a and ORF1b), the glycoproteins GP2 to GP5, the integral membrane protein M, and the nucleocapsid protein N (ORFs 2 to 7) [5].

References

Identification and characterization of a sixth structural protein of Lelystad virus: the glycoprotein GP2 encoded by ORF2 is incorporated in virus particles. Meulenberg, J.J., Petersen-den Besten, A. Virology (1996) [Pubmed]
Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture. Dobbe, J.C., van der Meer, Y., Spaan, W.J., Snijder, E.J. Virology (2001) [Pubmed]
Proteins encoded by open reading frames 3 and 4 of the genome of Lelystad virus (Arteriviridae) are structural proteins of the virion. van Nieuwstadt, A.P., Meulenberg, J.J., van Essen-Zanbergen, A., Petersen-den Besten, A., Bende, R.J., Moormann, R.J., Wensvoort, G. J. Virol. (1996) [Pubmed]
Baculovirus expression and immunological detection of the major structural proteins of porcine reproductive and respiratory syndrome virus. Kreutz, L.C., Mengeling, W.L. Vet. Microbiol. (1997) [Pubmed]
PRRSV, the virus. Meulenberg, J.J. Vet. Res. (2000) [Pubmed]

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