High impact information on Lrrn1

• Whole mount in situ hybridization against two genes of previously unknown embryonic expression, Lrrn1 and Musashi2, confirmed the selectivity of this screen for early neuroectodermal markers [1].
• Somitic expression occurs in the earliest myoblasts that originate from the dorsal lip in a pattern reminiscent of the muscle determination gene Myf5, but not at the ventral lip, indicating that NLRR-1 is expressed in a subset of myotome cells [2].
• The NLRR gene family and mouse development: Modified differential display PCR identifies NLRR-1 as a gene expressed in early somitic myoblasts [2].
• Using this technique, we isolated NLRR-1 as a gene expressed in the myotome of developing somites but not in the presomitic mesoderm [2].
• The NLRR genes comprise a three-gene family encoding glycosylated transmembrane proteins with external leucine-rich repeats, a fibronectin domain, an immunoglobulin domain and short intracellular tails capable of mediating protein-protein interaction [2].

Other interactions of Lrrn1

• We analyzed the whole sequence of NLRR-1 and partial sequence of NLRR-2 [3].

Analytical, diagnostic and therapeutic context of Lrrn1

• Sequence analysis showed that these two clones are about 60% homologous to each other, and that NLRR-1 protein is a transmembrane protein [3].
• Levels of expression of these NLRR mRNAs were measured in a unilateral cortical injury model by in-situ hybridization and Northern blot analysis [4].

References