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Gene Review

Y110A7A.6  -  Protein Y110A7A.6

Caenorhabditis elegans

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Disease relevance of phosphofructokinase

  • The complemented cells contain PFK enzyme activity, absent in the E. coli mutant, at levels considerably higher than in wild type E. coli [1].

High impact information on phosphofructokinase


Biological context of phosphofructokinase

  • Cloning and nucleotide sequence of a full-length cDNA encoding Ascaris suum phosphofructokinase [4].
  • However, several synthetic peptides whose sequence reflected the phosphorylation site of Ascaris suum phosphofructokinase (AKGRSDS*IV), or variations of it, were phosphorylated with the same efficiency by both protein kinases [5].
  • Analyses of the subunit primary structure reveal, as in other eukaryotic PFKs, that the amino-terminal half is homologous to the carboxy-terminal half, supporting the hypothesis that the PFK gene evolved by duplication of the prokaryotic gene and that the allosteric sites arose by mutations at the catalytic site [4].
  • An E. coli strain deleted for both PFK loci (strain DF1020) was transformed with plasmid DNA from a lambda ZAP II H. contortus cDNA library [1].
  • Phosphofructokinase (PFK), the key regulatory enzyme in glycolysis, has been cloned from the pathogenic parasitic nematode Haemonchus contortus by functional complementation in Escherichia coli [1].

Analytical, diagnostic and therapeutic context of phosphofructokinase


  1. Cloning of a cDNA encoding phosphofructokinase from Haemonchus contortus. Klein, R.D., Olson, E.R., Favreau, M.A., Winterrowd, C.A., Hatzenbuhler, N.T., Shea, M.H., Nulf, S.C., Geary, T.G. Mol. Biochem. Parasitol. (1991) [Pubmed]
  2. Phosphofructokinase from Dirofilaria immitis. Stimulation of activity by phosphorylation with cyclic AMP-dependent protein kinase. Srinivasan, N.G., Wariso, B.A., Kulkarni, G., Rao, G.S., Harris, B.G. J. Biol. Chem. (1988) [Pubmed]
  3. Serotonin receptors in parasitic worms. Mansour, T.E. Advances in parasitology. (1984) [Pubmed]
  4. Cloning and nucleotide sequence of a full-length cDNA encoding Ascaris suum phosphofructokinase. Kulkarni, G., Sabnis, N.A., Bhat, K.S., Harris, B.G. J. Parasitol. (2005) [Pubmed]
  5. Comparison of the substrate specificities of cAMP-dependent protein kinase from bovine heart and Ascaris suum muscle. Treptau, T., Piram, P., Cook, P.F., Rodriguez, P.H., Hoffmann, R., Jung, S., Thalhofer, H.P., Harris, B.G., Hofer, H.W. Biol. Chem. Hoppe-Seyler (1996) [Pubmed]
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