Disease relevance of mt-Nd5

• Transcripts of the mitochondrial gene ND5 are overexpressed in highly metastatic murine large cell lymphoma cells [1].

High impact information on mt-Nd5

• A further cDNA encompassing three mitochondrial genes was used as a probe to show that a third mRNA, NADH dehydrogenase subunit 5, was also down-regulated by interferon while a fourth, NADH dehydrogenase subunit 6, was unaffected [2].
• Tight control of respiration by NADH dehydrogenase ND5 subunit gene expression in mouse mitochondria [3].
• The derivation from this mutant of a large number of cell lines containing between 4 and 100% of the normal number of wild-type ND5 genes has allowed an analysis of the genetic and functional thresholds operating in mouse mitochondria [3].
• Most strikingly, the highest ND5 synthesis rate was just sufficient to support the maximum NADH dehydrogenase-dependent respiration rate, with no upregulation of translation occurring with decreasing wild-type mRNA levels [3].
• Transcription of other mitochondrial genes, including NADH-dehydrogenase subunit 5, is unregulated during trypanosome development [4].

Biological context of mt-Nd5

• The 1.2- and 2.4-kb RNAs coded by the H strand, putative mRNAs for cyt b and URF5 reading frames, respectively, are derived from a common precursor of 3.6-kb RNA [5].
• This gene was identified as ND5, which codes for a subunit of NADH dehydrogenase (complex I of the mitochondrial electron transport chain); this complex is the target for an activated macrophage-released cytostatic factor [6].
• Among the 13 genes examined, Nd5 produced the true tree in all tree-building methods or algorithms for both amino acid and nucleotide sequence data [7].
• Sequence homologies among mitochondrial DNA-coded URF2, URF4 and URF5 [8].

Anatomical context of mt-Nd5

• With this method, 15 mouse cell lines with deficiencies in complex I-dependent respiration were obtained, and two near-homoplasmic mutations in mouse ND5 and ND6 genes were isolated [9].

Other interactions of mt-Nd5

• In contrast, mRNAs coded by adjacently located reading frames with no interrupting tRNA genes such as URF3 -cyt ox III and URF5 -cyt b are processed from relatively long-lived precursors [10].

References