Disease relevance of TUBB

• Re: genetic analysis of the beta-tubulin gene, TUBB, in non-small-cell lung cancer [1].
• Indeed, M40 recombinants containing a functional PTAP motif specifically incorporated endogenous tsg101 into budding virions and were dependent on tsg101 expression for efficient budding [2].
• There are reported concerns that military personnel with EIB will have increased airway hyperreactivity or significant dyspnea while wearing the standard military M40 protective mask [3].
• The new M40/42 series protective mask has a different composition, and should be tried as an alternative to the M17 mask in service members with black rubber allergies [4].
• During a basic treatment with low-molecular dextrans (infukoll M40), 37 patients with an acute ischaemic cerebral stroke multiply underwent elevations in blood pressure up to systolic values of about 210 to 220 mmHg [5].

Psychiatry related information on TUBB

• In response to modifications of the visual surroundings the older subjects were 24%-47% (P < 0.05 and P < 0.001) slower in their response time in reaching the lit centre (TT) and remained 20%-34% (P < 0.001) less time inside the centre (TC) from the overall time of lighting than M40 and M20, respectively [6].

High impact information on TUBB

• Sequence analysis of parental and resistant cells revealed a single point mutation in the M40 isotype of beta-tubulin at nucleotide 1050 (G-->T, Lys(350)-->Asn) in the indanocine-resistant clone, in a region close to the putative colchicine binding site [7].
• A previously undescribed nonsynonymous single nucleotide polymorphism, encoding Ala(185)Thr, was identified in exon 4 of the beta-tubulin gene, TUBB, in three other patients [8].
• Two of the genes, M40 and beta 2, are expressed at varying levels in all of the cell lines examined, though the level of expression of one of these genes parallels the other in most cases [9].
• Two of the genes (M40 and 5 beta) contain one (M40) or ten (5 beta) members of the middle repetitive Alu family sequences within one of their intervening sequences [9].
• This system allowed us to isolate three HdV-specific cDNAs, termed M24, M27, and M40. cDNA nucleotide sequence analysis demonstrated significant regions of homology [10].

Biological context of TUBB

• The agonist profile of M40, a representative chimeric peptide, was found not to be the result of receptor reserve because receptor inactivation by partial alkylation experiments confirmed its full intrinsic efficacy under conditions of a "zero" reserve state [11].
• For the first time a cDNA encoding the membrane associated subunit M40 of an invertebrate V-ATPase has been isolated and sequenced, based on a cDNA library from larval midgut of the tobacco hornworm, Manduca sexta [12].
• No sequence variants in the exons of the beta-tubulin M40 gene were detected [13].
• The proposed BW respirator will be lightweight; will require low inhalation pressures; will be comfortable to wear for prolonged periods; will not interfere with vision, hearing, and communication; and will not degrade overall effectiveness and performance to the degree observed with the M17/M40 masks [14].

Anatomical context of TUBB

• Since M40 subunits had been identified only in endosomal V-ATPases till now, this result indicates that they are constitutive members of all, endomembrane and plasma membrane V-ATPases [12].
• M35, M40 and C7 showed high affinity for galanin receptors in the hypothalamus with Hill coefficients close to unity, whereas in the basal forebrain these peptides competed differently [15].
• The CSA of the quadriceps femoris increased in M40 by 5% (P < 0.05), in W40 by 9% (P < 0.01), in W70 by 6% (P < 0.05), and in M70 by 2% (not significant) [16].
• These results suggest that M20 and M40 to upper limb stimulation represent activation of distinct neuronal populations in hand SI cortex, presumably in area 3b [17].
• M20 was on average 7 mm more lateral along the central sulcus for median than ulnar nerve stimuli, in agreement with the somatotopic organization of the SI cortex; similar organization for M40 was less clear [17].

Associations of TUBB with chemical compounds

• Laulimalide was active against cell lines resistant to paclitaxel or epothilones A and B on the basis of mutations in the M40 human beta-tubulin gene [18].
• Gonadectomized, estradiol-treated male and female ferrets (Mustela furo) received intracerebroventricular (i.c.v.) infusions of 4 doses of the galanin receptor antagonist M40 or galanin and were allowed to approach breeding male or female ferrets that were placed behind wire mesh barriers in the goal boxes of a T maze [19].
• However, a novel point mutation (T to A) resulting in Asp26 to glutamate substitution in class I (M40) beta-tubulin was found [20].

Other interactions of TUBB

• Four single base substitutions were detected in the HSET gene, and none in the TUBB gene [21].

Analytical, diagnostic and therapeutic context of TUBB

• Immunoblotting with monospecific antibodies raised against the recombinant M40 polypeptide demonstrated that it is a subunit of the insect plasma membrane V-ATPase [12].
• The objective of this study is to determine whether the M40 protective gas mask increases airway hyperreactivity in military personnel with exertional dyspnea and the diagnosis of EIB [3].
• We therefore investigated the presence of mutations in the beta-tubulin M40 gene in 40 ovarian tumours (16 paraffin-embedded and 24 freshly frozen) selected for good or poor response to chemotherapy with paclitaxel or non-tubulin-affecting regimens [13].
• During the first night, subjects underwent standard polysomnography without the M40 mask [22].

References