High impact information on Slc16a7

• Recently, two monocarboxylate transporters, MCT1 and MCT2, have been cloned [1].
• These data provide convincing evidence that MCT2 represents a major neuronal monocarboxylate transporter in the adult mouse brain, and further suggest that mature neurons could use monocarboxylates such as lactate as additional energy substrates [2].
• Noradrenaline enhances monocarboxylate transporter 2 expression in cultured mouse cortical neurons via a translational regulation [3].
• Forskolin and dBcAMP also enhanced MCT2 expression, suggesting the implication of a cAMP-mediated pathway in the effect of NA [3].
• Treatment of cultured cortical neurons with 100 microm noradrenaline (NA) led, after a few hours, to a striking enhancement in fluorescence intensity associated with MCT2 IR in the cell soma as well as in dendrites [3].

Biological context of Slc16a7

• We have recently cloned two MCT isoforms (MCT1 and MCT2) from a mouse kidney cDNA library [4].

Anatomical context of Slc16a7

• Transcripts encoding for MCT1 and MCT2 were present, under a polyadenylated form, in the majority of the human and mouse oocytes and early embryos [5].
• It was observed that, at both the mRNA and the protein levels, astrocytes strongly expressed MCT1 but had very little if any MCT2 [6].
• MCT2 expression is more limited, with high levels of expression confined to testes, kidney, stomach, and liver and lower levels in lung, brain, and epididymal fat [4].
• In addition, MCT1 mRNA, but not MCT2, is localized to the choroid plexus, ependyma, microvessels, and white matter structures such as the corpus callosum [4].
• Among other MCT subtypes, only MCT2 was detected in the parietal cell region of the gastric mucosa [7].

Associations of Slc16a7 with chemical compounds

• Application of cycloheximide, a protein synthesis inhibitor, prevented the enhancement of MCT2 IR, while the mRNA synthesis inhibitor actinomycin D also blocked the effect of NA on MCT2 IR levels [3].

Other interactions of Slc16a7

• Parallel immunocytochemical stainings of cultured neurons with the presynaptic marker synaptophysin showed that MCT2 expression is correlated with synaptic development [6].

Analytical, diagnostic and therapeutic context of Slc16a7

• Western blot experiments performed on cultured neurons treated with NA confirmed that MCT2 protein expression was increased [3].
• We report here the characterization by Northern blot analysis and by in situ hybridization of the expression of MCT1 and MCT2 mRNAs in the mouse brain [1].
• Cell-specific localization of monocarboxylate transporters, MCT1 and MCT2, in the adult mouse brain revealed by double immunohistochemical labeling and confocal microscopy [8].
• Both MCT1 mRNA and MCT2 mRNA are detected in mouse brain using antisense riboprobes and in situ hybridization [4].

References