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Gene Review

Ofd1  -  oral-facial-digital syndrome 1 gene...

Mus musculus

Synonyms: Cxorf5, DXGgc7e, ORF2, Oral-facial-digital syndrome 1 protein homolog
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Disease relevance of Ofd1

  • These data suggest that the 2.0-kb LAT putative ORF1 and ORF2 (or the first 1,186 bp of the 2.0-kb LAT) are dispensable for explant reactivation of latent HSV-1 [1].
  • Two open reading frames (ORF1 and ORF2) within the 2.0-kilobase latency-associated transcript of herpes simplex virus type 1 are not essential for reactivation from latency [1].
  • We have expressed the second open reading frame of this gene (gene 5 ORF 2) in an Escherichia coli expression system [2].
  • Further analysis revealed that Orf2 is involved in preventing superinfection [3].
  • However, the precursor was processed to a 57 kDa protein and a 22 kDa polypeptide by the proteinase of feline calicivirus (FCV) when this was co-expressed with ORF2 [4].

High impact information on Ofd1

  • Mammalian LINEs have two ORFs (ORF1 and ORF2) [5].
  • Surprisingly, the skipped exon was inserted by the 7104 bp L1 element which carried the full-length stretch of the mouse L1 sequence, consisting of a 212 bp F-type tandem repeat, open reading frame 1 (ORF1), ORF2, the polyadenylation signal and a poly A stretch [6].
  • Predicted peptide sequence of ORF2 contained a zinc finger domain which showed significant homology to GC box binding proteins BTEB2 and SP1 [7].
  • Transfection of an expression vector containing ORF2 but not full length cDNA was able to transform NIH3T3 cells and induce tumors in nude mice [7].
  • ORF2 is capable of encoding the IAP integrase, an enzyme which catalyzes integration of proviral DNA into the genome [8].

Chemical compound and disease context of Ofd1


Biological context of Ofd1


Anatomical context of Ofd1


Associations of Ofd1 with chemical compounds


Analytical, diagnostic and therapeutic context of Ofd1

  • An antiserum to a synthetic peptide based on the IAP integrase gene sequence reacted with ORF2 product expressed in bacteria as a fusion protein, and detected a 47 kDa protein, predicted from the size of ORF2, in myeloma cell fractions by Western blotting [8].
  • In the present study, Northern blot and in situ hybridization analyses revealed a template-strand transcription of LINE-1 ORF2 (encoding reverse transcriptase, RT) in lymphoid organs and the liver from MRL-+/+ and Fas-deficient MRL/lpr strains and their normal ancestors [19].
  • The specificity of the antibody was verified by immunoprecipitation of the in vitro transcribed and translated protein product of gene 5 ORF 2 [2].
  • Anti-ORF-2 serum titers peaked at ca 1:5000 in the ELISA and 1:1000 in the Western blot [14].
  • The capsid (Cap) protein encoded by the PCV2 ORF2 gene may be an excellent candidate for vaccination [20].


  1. Two open reading frames (ORF1 and ORF2) within the 2.0-kilobase latency-associated transcript of herpes simplex virus type 1 are not essential for reactivation from latency. Fareed, M.U., Spivack, J.G. J. Virol. (1994) [Pubmed]
  2. Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame. Leibowitz, J.L., Perlman, S., Weinstock, G., DeVries, J.R., Budzilowicz, C., Weissemann, J.M., Weiss, S.R. Virology (1988) [Pubmed]
  3. Characterization of the major control region of Vibrio cholerae bacteriophage K139: immunity, exclusion, and integration. Nesper, J., Blass, J., Fountoulakis, M., Reidl, J. J. Bacteriol. (1999) [Pubmed]
  4. Expression and processing of the canine calicivirus capsid precursor. Matsuura, Y., Tohya, Y., Onuma, M., Roerink, F., Mochizuki, M., Sugimura, T. J. Gen. Virol. (2000) [Pubmed]
  5. In vitro properties of the first ORF protein from mouse LINE-1 support its role in ribonucleoprotein particle formation during retrotransposition. Kolosha, V.O., Martin, S.L. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  6. Dysfunction of the Orleans reeler gene arising from exon skipping due to transposition of a full-length copy of an active L1 sequence into the skipped exon. Takahara, T., Ohsumi, T., Kuromitsu, J., Shibata, K., Sasaki, N., Okazaki, Y., Shibata, H., Sato, S., Yoshiki, A., Kusakabe, M., Muramatsu, M., Ueki, M., Okuda, K., Hayashizaki, Y. Hum. Mol. Genet. (1996) [Pubmed]
  7. Identification of Bcd, a novel proto-oncogene expressed in B-cells. El Rouby, S., Newcomb, E.W. Oncogene (1996) [Pubmed]
  8. Characterization of amplified intracisternal A-particle elements encoding integrase. Lueders, K.K., Grossman, Z., Fewell, J.W. Nucleic Acids Res. (1989) [Pubmed]
  9. Elimination of antibiotic-resistant plasmids by quinolone antibiotics. Fu, K.P., Grace, M.E., Hsiao, C.L., Hung, P.P. Chemotherapy. (1988) [Pubmed]
  10. Nucleotide sequence of a marsupial LINE-1 element and the evolution of placental mammals. Dörner, M., Pääbo, S. Mol. Biol. Evol. (1995) [Pubmed]
  11. The L1 family of long interspersed repetitive DNA in rabbits: sequence, copy number, conserved open reading frames, and similarity to keratin. Demers, G.W., Matunis, M.J., Hardison, R.C. J. Mol. Evol. (1989) [Pubmed]
  12. A protein involved in stabilization of a large non-symbiotic plasmid of Rhizobium meliloti shows homology to eukaryotic cytoskeletal proteins and DNA-binding proteins. Mercado-Blanco, J., Olivares, J. Gene (1994) [Pubmed]
  13. Genomic organization of the mouse Msh4 gene producing bicistronic, chimeric and antisense mRNA. Hirano, M., Noda, T. Gene (2004) [Pubmed]
  14. DNA inoculation with a plasmid vector carrying the hepatitis E virus structural protein gene induces immune response in mice. He, J., Hoffman, S.L., Hayes, C.G. Vaccine (1997) [Pubmed]
  15. 5'UTR sequences of the glucocorticoid receptor 1A transcript encode a peptide associated with translational regulation of the glucocorticoid receptor. Diba, F., Watson, C.S., Gametchu, B. J. Cell. Biochem. (2001) [Pubmed]
  16. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice. Zhou, Y.X., Lee, M.Y., Ng, J.M., Chye, M.L., Yip, W.K., Zee, S.Y., Lam, E. World J. Gastroenterol. (2006) [Pubmed]
  17. Expression of Escherichia coli beta-galactosidase in Mycobacterium bovis BCG using an expression system isolated from Mycobacterium paratuberculosis which induced humoral and cellular immune responses. Murray, A., Winter, N., Lagranderie, M., Hill, D.F., Rauzier, J., Timm, J., Leclerc, C., Moriarty, K.M., Gheorghiu, M., Gicquel, B. Mol. Microbiol. (1992) [Pubmed]
  18. Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. Nishikawa, Y., Kimura, M., Xuan, X., Makala, L., Nagasawa, H., Mikami, T., Otsuka, H. Virus Res. (2002) [Pubmed]
  19. Unusual expression of LINE-1 transposable element in the MRL autoimmune lymphoproliferative syndrome-prone strain. Benihoud, K., Bonardelle, D., Soual-Hoebeke, E., Durand-Gasselin, I., Emilie, D., Kiger, N., Bobé, P. Oncogene (2002) [Pubmed]
  20. Construction and immunogenicity of recombinant adenovirus expressing the capsid protein of porcine circovirus 2 (PCV2) in mice. Wang, X., Jiang, W., Jiang, P., Li, Y., Feng, Z., Xu, J. Vaccine (2006) [Pubmed]
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