High impact information on Alpi

• IAP-I was also induced by sodium arsenite, which generates reactive oxygen species and is an inducer of members of the HSP family [1].
• Glutathione suppressed activating protein-1 and cAMP response element-binding protein activation caused by heat shock but did not suppress the expression of IAP-I [1].
• LPS instillation induced IAP-I mRNA, but not IAP-II mRNA or TNAP mRNA [2].
• There is a potential CAAT-box 14 bp 5' to the transcriptional start site, 36 bp upstream from IAP-I, and a TATA-box 31 bp 5' to the transcriptional start site, 55 bp upstream from IAP-II [3].
• Rat intestinal alkaline phosphatases (IAP-I and -II) differ in primary structure, substrate specificity, tissue localization, and response to fat feeding [3].

Biological context of Alpi

• Transfection of these promoter regions (linked to luciferase as a reporter gene) into a kidney cell line, COS-7, produced the differential response to oleic acid expected from in vivo studies, i.e., threefold increase using the 5' flanking region of IAP-II, but not IAP-I [3].

References