Disease relevance of Ppp2ca

• The mRNA level of PP2C alpha was decreased in the drug-resistant hepatomas compared with the parental drug-sensitive hepatomas, whereas mRNA levels of PP1 alpha, PP1 gamma 1 and PP2A alpha in resistant hepatomas showed diverse deviations, which are not drug-resistance-specific [1].

High impact information on Ppp2ca

• The cysteine at residue 269 present only in highly OA-sensitive protein serine/threonine phosphatase catalytic subunit isozymes, PP2A alpha, PP2A beta, and PPX, is suggested to be involved in the binding of OA [2].
• Nucleotide sequencing of PP2A alpha (an isotype of PP2A catalytic subunit) cDNA demonstrated that both variants have a T-->G transversion at the first base of codon 269 (805 nt), which results in substitution of glycine for cysteine [2].
• We expressed in COS-1 cells a mutant PP2A alpha tagged with the influenza hemagglutinin epitope [2].
• It is suggested that the PP2A alpha mutation in cooperation with a high level of P-glycoprotein makes the CHO-K1 variants highly resistant to OA [2].
• The recombinant mutant PP2A alpha protein immunoprecipitated with an anti-influenza hemagglutinin antibody was more resistant than the wild type to OA, their IC50 values being 0.65 nM and 0.15 nM, and their IC80 values being 4.0 nM and 0.45 nM, respectively [2].

Biological context of Ppp2ca

• We speculate on the role of PP2A alpha-mediated dephosphorylation in cardiac morphogenesis and suggest a number of possible molecular targets [3].
• The gene expression for alpha and beta isoforms of type 2A protein phosphatase (PP2A-alpha and -beta) in the adult rat brain was examined by in situ hybridization analysis [4].

Anatomical context of Ppp2ca

• Cardiac PP2A alpha expression was regionally restricted to the atrium, ventricle, and truncus arteriosus [3].
• At term, PP2A alpha was highly expressed in endothelial cells, but not in the underlying myocardium [3].
• In situ hybridization on embryonic d 12 showed that PP2A alpha mRNA was expressed in the heart, brain, tail, and limb buds [3].
• Cross-sectional hybridization revealed PP2A alpha mRNA in the epicardium, pericardium, and endothelium [3].

Associations of Ppp2ca with chemical compounds

• They were proven to have a mutation in the PP2A alpha catalytic subunit, in which cysteine 269 had been substituted for glycine; and it was demonstrated that this region interacts with OA [5].

Analytical, diagnostic and therapeutic context of Ppp2ca

• In this study, we examined the developmental expression of PP2A alpha mRNA and protein in the heart using Northern and Western analysis, in situ hybridization, and immumohistochemical staining [3].
• By Western blotting, PP2A alpha protein levels were twice as high in the embryonic rat heart compared with the adult [3].

References