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Gene Review

Sry  -  sex determining region Y

Rattus norvegicus

Synonyms: SRYGENE, Sex-determining region Y protein, Sry1, Sry3BI, Tdf, ...
 
 
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High impact information on Sry

  • A PCR specific for the Y-chromosome (sex-determining region Y [Sry]) allowed the distinction of small amounts of male cells in a large excess of female cells [1].
  • Our results indicate that the Sry-specific PCR is useful for assessing microchimerism following rat liver transplantation, and demonstrate correlation between microchimerism and graft outcome; high-level microchimerism (>O.l percent) correlated well with graft acceptance, whereas low-level microchimerism (<O.01 percent) did not [1].
  • Furthermore, using antisense oligodeoxynucleotides, we show that Sry downregulation in the substantia nigra causes a statistically significant decrease in tyrosine hydroxylase expression with no overall effect on neuronal numbers and that this decrease leads to motor deficits in male rats [2].
  • Next, 2 x 10(5) cells of each type were transplanted into female liver regenerative model dipeptidyl peptidase-deficient rats, and we estimated the rate of liver repopulation by the transplanted hepatocytes in the host liver, as determined by recognition of the Sry gene on the Y-chromosome [3].
  • In this study, we reconstitute the nuclear import of SRY in vitro, demonstrating a lack of requirement for exogenous factors for nuclear accumulation and a significant reduction in nuclear transport in the presence of antibodies to importin beta but not importin alpha [4].
 

Biological context of Sry

 

Anatomical context of Sry

  • The Sry-related protein Sox10 is selectively expressed in neural crest cells during early stages of development and in glial cells of the peripheral and central nervous systems during late development and in the adult [9].
  • Superficial epithelial cells were sampled weekly, and the DNA extracted and probed for the male-specific gene Sry by polymerase chain reaction [7].
  • These Sertoli-like cells express, however, the female-specific marker FOXL2 (Forkhead L2) but not the male sex-specific marker SOX-9 (Sry-type high-mobility-group box transcription factor-9) underlying the maintenance of molecular characteristics of granulosa cells [10].
  • Co-transfection of PC12 cells with expression vector for Sry and the reporter construct [p5'TH(-773/+27)/Luc], containing 773 of the proximal nucleotides of the TH promoter directing luciferase reporter activity, led to elevation of reporter activity [8].
  • Y-chromosome-specific sequence (Sry) of male SD rats could be detected in the bone marrow, spleen and thymus of female recipients at 15 d after bone marrow infusion [11].
 

Regulatory relationships of Sry

  • Sry gene-induced development of testes constitutes a binary switch directing all subsequent differentiation [12].
  • Here we tested the hypothesis that Sry regulates transcription of tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of catecholamines [8].
 

Other interactions of Sry

 

Analytical, diagnostic and therapeutic context of Sry

References

  1. Assessment of microchimerism in rat liver transplantation by polymerase chain reaction. Tashiro, H., Fukuda, Y., Kimura, A., Hoshino, S., Ito, H., Dohi, K. Hepatology (1996) [Pubmed]
  2. Direct regulation of adult brain function by the male-specific factor SRY. Dewing, P., Chiang, C.W., Sinchak, K., Sim, H., Fernagut, P.O., Kelly, S., Chesselet, M.F., Micevych, P.E., Albrecht, K.H., Harley, V.R., Vilain, E. Curr. Biol. (2006) [Pubmed]
  3. Effective hepatocyte transplantation using rat hepatocytes with low asialoglycoprotein receptor expression. Ise, H., Nikaido, T., Negishi, N., Sugihara, N., Suzuki, F., Akaike, T., Ikeda, U. Am. J. Pathol. (2004) [Pubmed]
  4. The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1. Forwood, J.K., Harley, V., Jans, D.A. J. Biol. Chem. (2001) [Pubmed]
  5. Use of a rat cDNA probe specific for the Y chromosome to detect male-derived cells. An, J., Beauchemin, N., Albanese, J., Abney, T.O., Sullivan, A.K. J. Androl. (1997) [Pubmed]
  6. Peripheral nerve allografts stored in green tea polyphenol solution. Ikeguchi, R., Kakinoki, R., Matsumoto, T., Hyon, S.H., Nakamura, T. Transplantation (2005) [Pubmed]
  7. Effect of immunosuppression on outcome measures in a model of rat limbal transplantation. Mills, R.A., Coster, D.J., Williams, K.A. Invest. Ophthalmol. Vis. Sci. (2002) [Pubmed]
  8. Regulation of tyrosine hydroxylase gene transcription by Sry. Milsted, A., Serova, L., Sabban, E.L., Dunphy, G., Turner, M.E., Ely, D.L. Neurosci. Lett. (2004) [Pubmed]
  9. Functional analysis of Sox10 mutations found in human Waardenburg-Hirschsprung patients. Kuhlbrodt, K., Schmidt, C., Sock, E., Pingault, V., Bondurand, N., Goossens, M., Wegner, M. J. Biol. Chem. (1998) [Pubmed]
  10. Follicular cells acquire sertoli cell characteristics after oocyte loss. Guigon, C.J., Coudouel, N., Mazaud-Guittot, S., Forest, M.G., Magre, S. Endocrinology (2005) [Pubmed]
  11. Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation. Xie, H.Y., Huang, D.S., Jia, C.K., Zheng, S.S. Journal of Zhejiang University. Science. B. (2005) [Pubmed]
  12. Molecular aspects of sexual differentiation of the rodent brain. McCarthy, M.M. Psychoneuroendocrinology (1994) [Pubmed]
  13. X-chromosomal localization of mammalian Y-linked genes in two XO species of the Ryukyu spiny rat. Arakawa, Y., Nishida-Umehara, C., Matsuda, Y., Sutou, S., Suzuki, H. Cytogenet. Genome Res. (2002) [Pubmed]
  14. Hepatic potential of bone marrow stromal cells: development of in vitro co-culture and intra-portal transplantation models. Luk, J.M., Wang, P.P., Lee, C.K., Wang, J.H., Fan, S.T. J. Immunol. Methods (2005) [Pubmed]
  15. Spleen dendritic cells of recipients of allogeneic but not syngeneic heart grafts internalize donor DNA fragments. Interewicz, B., Olszewski, W.L., Maksymowicz, M., Stanislawska, J., Szyper, E. Ann. Transplant. (2004) [Pubmed]
  16. Experimental study on hepatic reinnervation after orthotopic liver transplantation in rats. Sakamoto, I., Takahashi, T., Kakita, A., Hayashi, I., Majima, M., Yamashina, S. J. Hepatol. (2002) [Pubmed]
 
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