Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

AAT1 - Aortic aneurysm, familial thoracic 1

Homo sapiens

Hasannia, S. et al., Martins, L.L. et al.

Martins, Mourato, de Varennes,

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High impact information on AAT1

AAT-2 uses 2-amino-4-methoxy-4-oxobutanoic acid at a similar rate as L-aspartate but AAT-1 uses this substrate at a slower rate [1].
The chimeric exon-intron complex in the middle of the naturally occurring hAAT exons II and III caused a 23-fold enhancement of hAAT expression in P. pastoris, measured through SDS-PAGE and immunoblot analyses [2].
To address this, human Alpha-1 antitrypsin (hAAT) gene was integrated into Pichia pastoris with and without a yeast intron generated from the final plasmid pBlu-exII-int-exIII and ligated into the EcoRI/BamHI multiple cloning site of the yeast shuttle vector pHIL-S1 [2].

References

Effects of substrate structural analogues on the enzymatic activities of aspartate aminotransferase isoenzymes. Martins, L.L., Mourato, M.P., de Varennes, A. J. Enzym. Inhib. (2001) [Pubmed]
Elevated expression of human alpha-1 antitrypsin mediated by yeast intron in Pichia pastoris. Hasannia, S., Lotfi, A.S., Mahboudi, F., Rezaii, A., Rahbarizadeh, F., Mohsenifar, A. Biotechnol. Lett. (2006) [Pubmed]

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