Disease relevance of ade6

• Quantitative comparison of MNase sensitivity of the chromatin in premeiotic and meiotic cultures revealed a small meiotic induction of MNase hypersensitivity in the ade6 promoter region of the wild-type and M375 strains [1].
• 12 organophosphorus insecticides were tested for toxicity and mutagenicity in the forward mutation test system ade6 of the yeast Schizosaccharomyces pombe [2].

High impact information on ade6

• Placement of the ade6 gene within cen1 or cen3 resulted in red-white sectored colonies, demonstrating the instability of gene expression [3].
• The meiotic induction of MNase hypersensitivity was enhanced significantly in the ade6 promoter region of the M26 strain and also occurred at the M26 mutation site [1].
• The G -->T transversion mutation, ade6-M26, creates the heptanucleotide sequence ATGACTG, which lies close to the 5' end of the open reading frame of the ade6 gene in Schizosaccharomyces pombe [1].
• The rec10 mutations are recessive and are on chromosome I; the ade6 region is on chromosome III [4].
• Similar ade6 regional specificities were observed for rec8 and rec11 [4].

Biological context of ade6

• DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination host spot allele ade6-M26 [5].
• The gene ade6 is located on chromosome III of the fission yeast Schizosaccharomyces pombe [5].
• It complements ade6 mutations when present on plasmids [5].
• Strains carrying these deletions of lys2(+) or his7(+) serve as relatively efficient hosts for the deletion of the ade6(+) gene by lys2(+)-- or his7(+)--marked PCR products when compared with hosts carrying lys2 or his7 point mutations [6].
• Truncated minichromosome derivatives were recovered containing the ura4 or ade6 gene adjacent to a newly formed telomere [7].

Associations of ade6 with chemical compounds

• The ade6 gene was chosen as a target as mutations within the gene confer adenine auxotrophy and a red colony phenotype, and it was expected that antisense or ribozyme RNA-mediated mutant phenocopies would exhibit the same readily detectable phenotype [8].
• We have begun a genetic investigation into the nature and cause of pigmentation of ade6 mutants of S. pombe and have discovered that one of these pigmentation defective mutants, apd1 (adenine pigmentation defective), is a strict glutathione auxotroph [9].

Other interactions of ade6

• These telomeres exert reversible position effects on the expression of the adjacent ura4 or ade6 genes [7].
• A 700-bp fragment containing the functional adh1 promoter was used to replace the weak wild-type promoter of the ade6 gene [10].
• This bias was observed in conversion events at three other loci, ade6, ade7, and ura1 [11].
• The effect of the strong promoter from the alcohol dehydrogenase gene on mitotic and meiotic intragenic recombination has been studied at the ade6 locus of the fission yeast Schizosaccharomyces pombe [10].

References