The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

  • Homologues
  • NCBI Gene
  • NCBI SNP
  • iHOP resource
  • SNPedia

Table of contents

About

Terms

 

Gene Review: 

rpb3  -  RNA polymerase II subunit 3

Schizosaccharomyces pombe 972h-

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on rpb3

  • Using the degenerated primers designed after amino acid micro-sequencing of the 40 kDa third-largest polypeptide (subunit 3), we cloned the subunit 3 gene (rpb3) and determined its DNA sequence [1].
  • Taken together with the sequence of parts of PCR-amplified cDNA, the predicted coding sequence of rpb3, interrupted by two introns, was found to encode a polypeptide of 297 amino acid residues in length with a molecular weight of 34 kDa [1].
  • The protein-to-mRNA ratio or the translation efficiency is low for the rpb1, rpb2, rpb3 and rpb11 genes, encoding the homologues of subunits beta', beta, alpha and alpha, respectively, of the prokaryotic RNA polymerase core enzyme [2].
  • To gain an insight into the roles of subunit 3, the eukaryotic homologue of alpha, temperature-sensitive mutants of the fission yeast, Schizosaccharomyces pombe, have been isolated after transformation of the mutagenized rpb3 gene encoding mutant subunit 3 of RNA polymerase II [3].
  • For the isolation of subunit complexes formed in the virus-infected cells, a glutathione S -transferase (GST) sequence was fused to the rpb3 cDNA to produce GST-Rpb3 fusion protein and a decahistidine-tag sequence was inserted into the rpb1 cDNA to produce Rpb1H protein [4].

References

  1. Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene. Azuma, Y., Yamagishi, M., Ishihama, A. Nucleic Acids Res. (1993) [Pubmed]
  2. Transcription organization and mRNA levels of the genes for all 12 subunits of the fission yeast RNA polymerase II. Sakurai, H., Ishihama, A. Genes Cells (2001) [Pubmed]
  3. Isolation of thermolabile mutant RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene. Azuma, Y., Yasui, K., Yamagishi, M., Ishihama, A. J. Biochem. (1995) [Pubmed]
  4. Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II. Kimura, M., Ishihama, A. Nucleic Acids Res. (2000) [Pubmed]
Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities