High impact information on cyr1

• Combination of the cyr1 and sxa2 mutations enhanced this susceptibility [1].
• P-factor induced not only responses toward mating but also arrest of the cell cycle at the G1 phase in h- cyr1 sxa2 cells [1].
• These phenotypes mimic those of cells defective in cyr1 encoding adenylyl cyclase [2].
• In this report we demonstrate that one of these genes, git2, is the same as the cyr1 gene, which encodes adenylate cyclase, and that loss-of-function mutations in git2 cause constitutive fbp1 transcription [3].
• A greater than 10-fold increase in the amount of cyr1 mRNA was observed when cloned cyr1+ was introduced into Sch. pombe cells on a multicopy plasmid [4].

Biological context of cyr1

• Here, we show that gpa2 exhibits a direct and GTP-dependent binding to the Ras-associating domain (RAD) of cyr1, which is identified by a computer algorithm-based search of the cyr1 amino acid sequence [5].
• Six ORFs correspond to the previously known rec14+, tug1+, rum1+, pch1+, gpd1+ and cyr1+ genes [6].
• When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos [7].

Associations of cyr1 with chemical compounds

• Cyclic AMP was detected in all nine sam mutant cells, and over-expression of the adenylyl cyclase gene (cyr1) failed to suppress the hyper-sporulation phenotype of these sam mutants, suggesting that none of the sam mutants were likely to be allelic to cyr1 [8].

References