Disease relevance of pck1

• The pck1-deletion partially rescued beta-glucanase hypersensitivity of the ksg1-overexpressing strain but the pck2-deletion increased it [1].

High impact information on pck1

• Two novel protein kinase C (PKC)-like genes, pck1+ and pck2+ were isolated from fission yeast by PCR [2].
• Gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability [2].
• Thus, overexpression of pck2(+), but not pck1(+), caused a general increase in cell wall biosynthesis, mainly in beta-glucan, and (1-3)beta-D-glucan synthase activity was considerably augmented [3].
• In addition, pck2p, but not pck1p, seems to be involved in keeping cell polarity [3].

Biological context of pck1

• Genetic complementation assays showed that an overexpression of pck2, the homologue of pck1 involved in the regulation of cell wall synthesis, could partially rescue ksg1-358 phenotypes [1].

Anatomical context of pck1

• Although overexpression of rho2(+) in wild-type or pck1 delta cells is lethal and causes morphological alterations, actin depolarization, and an increase in alpha-D-glucan biosynthesis, all of these effects are suppressed in a pck2 delta strain [4].

References