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Hck  -  hemopoietic cell kinase

Rattus norvegicus

Synonyms: Hctk, Hematopoietic cell kinase, Hemopoietic cell kinase, Tyrosine-protein kinase HCK, p56-HCK, ...
 
 
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Disease relevance of Hck

  • Recent studies have shown that high affinity interaction of the SH3 domain of Hck with the human immunodeficiency virus type I Nef protein activates Hck tyrosine kinase and biological activities, suggesting a mechanism that involves disruption of the SH3-linker interaction [1].
  • SH3-mediated Hck tyrosine kinase activation and fibroblast transformation by the Nef protein of HIV-1 [2].
 

High impact information on Hck

 

Biological context of Hck

 

Anatomical context of Hck

  • To test the role of this interaction in the regulation of Hck kinase activity in living cells, we substituted alanines for prolines 225 and 228 in the linker region and observed that the resulting mutant (Hck-2PA) demonstrated strong transforming activity in a Rat-2 fibroblast focus-forming assay [1].
  • These results provide direct evidence that SH3 engagement is sufficient to activate a Src family kinase in vivo and suggest that Hck may be activated by Nef in HIV-infected macrophages [2].
  • Enhanced transformation correlated with increased Hck/Nef complex formation at the plasma membrane [6].
  • Hybridoma cell lines were established that secrete monoclonal antibodies (MAbs) to Hck [7].
  • Hck, a member of the Src-family of protein tyrosine kinases, is expressed primarily in hematopoietic cells of the myeloid and B-lymphocyte lineages [7].
 

Associations of Hck with chemical compounds

  • Hck was detected in the eluate of WGA-Sepharose column, suggesting that it interacts with WGA binding glycoprotein (s) [5].
  • When these fusion proteins (or GST), immobilized on glutathione-agarose beads were incubated with [35S] methionine labelled cell extracts, multiple proteins which interact specifically with SH3 domain of Hck were detected by SDS-PAGE followed by autoradiography [5].
 

Other interactions of Hck

  • Hck is a member of the Src protein-tyrosine kinase family and is expressed strongly in macrophages, an important HIV target cell [6].
 

Analytical, diagnostic and therapeutic context of Hck

  • Activation of Hck, an Src family kinase, was analyzed by Western blot using a phosphospecific antibody [8].

References

  1. SH2-kinase linker mutations release Hck tyrosine kinase and transforming activities in Rat-2 fibroblasts. Briggs, S.D., Smithgall, T.E. J. Biol. Chem. (1999) [Pubmed]
  2. SH3-mediated Hck tyrosine kinase activation and fibroblast transformation by the Nef protein of HIV-1. Briggs, S.D., Sharkey, M., Stevenson, M., Smithgall, T.E. J. Biol. Chem. (1997) [Pubmed]
  3. Activation of the Src family kinase Hck without SH3-linker release. Lerner, E.C., Trible, R.P., Schiavone, A.P., Hochrein, J.M., Engen, J.R., Smithgall, T.E. J. Biol. Chem. (2005) [Pubmed]
  4. Physical and functional interaction between Hck tyrosine kinase and guanine nucleotide exchange factor C3G results in apoptosis, which is independent of C3G catalytic domain. Shivakrupa, R., Radha, V., Sudhakar, C.h., Swarup, G. J. Biol. Chem. (2003) [Pubmed]
  5. Interaction of SH3 domain of Hck tyrosine kinase with cellular proteins containing proline-rich regions: evidence for modulation by unique domain. Gouri, B.S., Swarup, G. Indian J. Biochem. Biophys. (1997) [Pubmed]
  6. Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck. Ye, H., Choi, H.J., Poe, J., Smithgall, T.E. Biochemistry (2004) [Pubmed]
  7. Generation and characterization of monoclonal antibodies to the Src-family kinase Hck. Cartledge, K., Dunn, A.R., Scholz, G. Hybridoma (2000) [Pubmed]
  8. The role of the Src family of tyrosine kinases after oxidant-induced lung injury in vivo. Khadaroo, R.G., He, R., Parodo, J., Powers, K.A., Marshall, J.C., Kapus, A., Rotstein, O.D. Surgery (2004) [Pubmed]
 
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