The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

Poli  -  polymerase (DNA directed), iota

Mus musculus

Synonyms: DNA polymerase iota, Rad30 homolog B, Rad30b
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on Poli

  • Here, we generated Polh Poli double-deficient mice and compared the tumor susceptibility of them with Polh- or Poli-deficient animals under the same genetic background [1].
  • Consequently, the Poli is an intriguing Par2 candidate clearly deserving further evaluation [2].
  • The mouse homolog (Rad30b) is located on chromosome 18E2 [3].
  • In situ hybridization analysis indicates that expression of mouse Rad30b occurs predominantly in postmeiotic round spermatids [3].
  • Although the frequency of mutations was decreased in the BL2 cell line deficient for polymerase iota, hypermutation was normal in the 129 strain of mice, which has a natural nonsense mutation in the Poli gene [4].
 

Biological context of Poli

  • Among those, the Poli seemed to be the most reasonable Par2 candidate, since it encodes an extremely error-prone DNA polymerase preferentially incorporating G or T opposite template T in vitro, reminiscent of the Kras2 activation because of an A to G or T point mutation within codon 61 with which most urethane-induced lung tumors are initiated [2].
 

Anatomical context of Poli

  • It is shown that the extracts of the brain and testis cells exhibit the highest activity of DNA polymerase iota, which is not in agreement with the results of other authors [5].
 

Associations of Poli with chemical compounds

  • Using homogeneous preparations of the enzyme, it was shown previously that DNA polymerase iota incorporated preferentially dGMP opposite the thymidine of the template in the growing DNA chain [5].

References

  1. UV-B Radiation Induces Epithelial Tumors in Mice Lacking DNA Polymerase {eta} and Mesenchymal Tumors in Mice Deficient for DNA Polymerase {iota}. Ohkumo, T., Kondo, Y., Yokoi, M., Tsukamoto, T., Yamada, A., Sugimoto, T., Kanao, R., Higashi, Y., Kondoh, H., Tatematsu, M., Masutani, C., Hanaoka, F. Mol. Cell. Biol. (2006) [Pubmed]
  2. Analysis of lung tumorigenesis in chimeric mice indicates the Pulmonary adenoma resistance 2 (Par2) locus to operate in the tumor-initiation stage in a cell-autonomous manner: detection of polymorphisms in the Poli gene as a candidate for Par2. Lee, G.H., Nishimori, H., Sasaki, Y., Matsushita, H., Kitagawa, T., Tokino, T. Oncogene (2003) [Pubmed]
  3. Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta. McDonald, J.P., Rapić-Otrin, V., Epstein, J.A., Broughton, B.C., Wang, X., Lehmann, A.R., Wolgemuth, D.J., Woodgate, R. Genomics (1999) [Pubmed]
  4. Normal hypermutation in antibody genes from congenic mice defective for DNA polymerase iota. Martomo, S.A., Yang, W.W., Vaisman, A., Maas, A., Yokoi, M., Hoeijmakers, J.H., Hanaoka, F., Woodgate, R., Gearhart, P.J. DNA Repair (Amst.) (2006) [Pubmed]
  5. DNA polymerase iota-like activity in crude cell extracts of different mouse organs. Gening, L.V., Petrochenkov, A.N., Reshetnyak, A.B., Andreeva, L.E., Tarantul, V.Z. Biochemistry Mosc. (2004) [Pubmed]
 
WikiGenes - Universities