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Gene Review:

xis - Excisionase

Enterobacteria phage lambda

Peredelchuk, M.Y. et al., Abremski, K. et al., Cho, E.H. et al., Sam, M.D. et al.

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Disease relevance of xis

After transduction of each copy, a helper plasmid bearing phage lambda xis and int genes is introduced into the cells to excise the drug resistance gene flanked with the lambda attL and lambda attR sites from the chromosome [1].
Our results demonstrate that E. coli lac promoter and lambda pL promoter fusions to the xis gene produce high levels of Xis protein [2].
Crystallization, dehydration and preliminary X-ray analysis of excisionase (Xis) proteins cooperatively bound to DNA [3].

High impact information on xis

The bacteriophage lambda excisionase (Xis) is a sequence-specific DNA binding protein required for excisive recombination [4].
In addition, one of these plasmids allows the control of xis expression in vivo [2].

References

A method for construction of E. coli strains with multiple DNA insertions in the chromosome. Peredelchuk, M.Y., Bennett, G.N. Gene (1997) [Pubmed]
Escherichia coli plasmid vectors for high-level regulated expression of the bacteriophage lambda xis gene product. Abremski, K., Hoess, R. Gene (1983) [Pubmed]
Crystallization, dehydration and preliminary X-ray analysis of excisionase (Xis) proteins cooperatively bound to DNA. Sam, M.D., Abbani, M.A., Cascio, D., Johnson, R.C., Clubb, R.T. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2006) [Pubmed]
Characterization of bacteriophage lambda excisionase mutants defective in DNA binding. Cho, E.H., Alcaraz, R., Gumport, R.I., Gardner, J.F. J. Bacteriol. (2000) [Pubmed]

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