High impact information on NEB

• Reduced concentration of IGF-I in serum during luteal development does not account for suppressed luteal growth and progesterone synthesis of heifers in NEB [1].
• Holstein heifers (approximately 1 yr old) were fed to be in negative energy balance (n = 14; NEB) or positive energy balance (n = 15; PEB) during four estrous cycles [1].
• The results showed that there was very little difference in the rate of postmortem degradation of the intact form of titin or of intact nebulin with respect to the two types of samples examined [2].
• Body condition at onset of NEB may determine when effects of NEB on progesterone are detected [3].
• MBC-NEB heifers had increased ratios of small to large luteal cells [3].

Anatomical context of NEB

• Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting comparisons of purified myofibrils and whole muscle preparations for evaluating titin and nebulin in postmortem bovine muscle [2].

Associations of NEB with chemical compounds

• We conclude that reduced concentrations of progesterone in serum of FBC-PEB and MBC-NEB heifers is due to impaired luteal development [3].
• Secretion of progesterone in vitro was increased by LH for PEB but not NEB heifers [3].

Other interactions of NEB

• Exogenous bST increased (P < .01) IGF-I in serum, but this increase was greater in heifers in PEB than those in NEB (P < .01 for interaction of energy balance and bST) [1].
• Independent of BC, NEB decreased concentrations of insulin and increased GH and NEFA [3].

Analytical, diagnostic and therapeutic context of NEB

• The MF and WM samples from all steers were analyzed by SDS-PAGE (5% gels) and by Western blot analysis using monoclonal antibodies to titin and nebulin [2].

References