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Gene Review

PDP1  -  pyruvate dehyrogenase phosphatase...

Bos taurus

Synonyms: PDP, PPM2C
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High impact information on PDP

  • It decreases the sensitivity of the catalytic subunit of PDP (PDPc) to Mg2+ [1].
  • The polyamine spermine increases the sensitivity of PDP but not PDPc to Mg2+, apparently by interacting with PDPr [1].
  • These findings suggest that PDPr may be involved in the insulin-induced activation of the mitochondrial PDP in adipose tissue, which is characterized by a decrease in its apparent Km for Mg2+ [1].
  • Bovine pyruvate dehydrogenase phosphatase (PDP) is a Mg2+-dependent and Ca2+-stimulated heterodimer that is a member of the protein phosphatase 2C family and is localized to mitochondria [1].
  • At the other end of the lipoyl domain, substitution of Glu(182) by alanine or glutamine removed L2 binding to PDP1 or PDP1c, and these substitutions for the neighboring Glu(179) also greatly hindered complex formation (E179A > E179Q) [2].

Biological context of PDP


Anatomical context of PDP


Associations of PDP with chemical compounds

  • Thus, pyruvate dehydrogenase complex activity is increased by enhanced availability of PDP to its E2-bound, phosphorylated pyruvate dehydrogenase substrate as a consequence of the Ca2+-facilitated interchange of PDP among the mobile L2 domains and an essential (undetermined) step engaging the L2 lipoate [11].
  • Affinity-bound glutathione S-transferase-L2 was used to purify PDP to homogeneity by selective binding and elution by Ca2+ chelation [11].
  • The NHS group was reacted with an aminolipid for liposome anchorage, and antibodies were bound to the PDP group via disulfide bonds [12].
  • The milk is extracted with chloroform, the chloroform is evaporated, and the residue is suspended in hexane and extracted with 0.1M KH2PO4 (PDP) solution [13].
  • This antagonist, termed fraction V3, is now shown to inhibit the action of an inositol glycan insulin pH 2.0 mediator that stimulates pyruvate dehydrogenase phosphatase in a similar manner to insulin [5].

Other interactions of PDP

  • Thus, DARPP-32 appears to be a component of a regulatory cascade of phosphatases in which dephosphorylation of Ser-136 by protein phosphatase 2C facilitates dephosphorylation of Thr-34 by calcineurin, removing the cyclic nucleotide-induced inhibition of protein phosphatase 1 [14].

Analytical, diagnostic and therapeutic context of PDP


  1. Role of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase. Yan, J., Lawson, J.E., Reed, L.J. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  2. Structural requirements within the lipoyl domain for the Ca2+-dependent binding and activation of pyruvate dehydrogenase phosphatase isoform 1 or its catalytic subunit. Turkan, A., Gong, X., Peng, T., Roche, T.E. J. Biol. Chem. (2002) [Pubmed]
  3. Molecular cloning and expression of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase and sequence similarity with protein phosphatase 2C. Lawson, J.E., Niu, X.D., Browning, K.S., Trong, H.L., Yan, J., Reed, L.J. Biochemistry (1993) [Pubmed]
  4. ZMPP2, a novel type-2C protein phosphatase from maize. Broz, A.K., Thelen, J.J., Muszynski, M.G., Miernyk, J.A., Randall, D.D. J. Exp. Bot. (2001) [Pubmed]
  5. Circulating factors and insulin resistance. II. The action of the novel myo-inositol cyclic 1,2-inositol phosphate phosphoglycan insulin antagonist from human plasma in regulating pyruvate dehydrogenase phosphatase. Galasko, G.T., Abe, S., Lilley, K., Zhang, C., Larner, J. J. Clin. Endocrinol. Metab. (1996) [Pubmed]
  6. The multifunctional role of hormone-sensitive lipase in lipid metabolism. Yeaman, S.J., Smith, G.M., Jepson, C.A., Wood, S.L., Emmison, N. Adv. Enzyme Regul. (1994) [Pubmed]
  7. Changes in poly(A) tail length of maternal transcripts during in vitro maturation of bovine oocytes and their relation with developmental competence. Brevini-Gandolfi, T.A., Favetta, L.A., Mauri, L., Luciano, A.M., Cillo, F., Gandolfi, F. Mol. Reprod. Dev. (1999) [Pubmed]
  8. Functional characterization and localization of protein phosphatase type 2C from Paramecium. Grothe, K., Hanke, C., Momayezi, M., Kissmehl, R., Plattner, H., Schultz, J.E. J. Biol. Chem. (1998) [Pubmed]
  9. Role of protein phosphatase 2C from bovine adrenal chromaffin cells in the dephosphorylation of phospho-serine 40 tyrosine hydroxylase. Bevilaqua, L.R., Cammarota, M., Dickson, P.W., Sim, A.T., Dunkley, P.R. J. Neurochem. (2003) [Pubmed]
  10. Molecular cloning and characterization of protein phosphatase 2C of vomeronasal sensory epithelium of garter snakes. Wang, D., Liu, W., Liu, J., Chen, P., Quan, W., Halpern, M. Arch. Biochem. Biophys. (2002) [Pubmed]
  11. Activated function of the pyruvate dehydrogenase phosphatase through Ca2+-facilitated binding to the inner lipoyl domain of the dihydrolipoyl acetyltransferase. Chen, G., Wang, L., Liu, S., Chuang, C., Roche, T.E. J. Biol. Chem. (1996) [Pubmed]
  12. Synthesis and applications of a new poly(ethylene glycol) derivative for the crosslinking of amines with thiols. Haselgrübler, T., Amerstorfer, A., Schindler, H., Gruber, H.J. Bioconjug. Chem. (1995) [Pubmed]
  13. Liquid chromatographic method for determination of sulfamethazine residues in milk: collaborative study. Weber, J.D., Smedley, M.D. Journal of AOAC International. (1993) [Pubmed]
  14. Dephosphorylation of Ser-137 in DARPP-32 by protein phosphatases 2A and 2C: different roles in vitro and in striatonigral neurons. Desdouits, F., Siciliano, J.C., Nairn, A.C., Greengard, P., Girault, J.A. Biochem. J. (1998) [Pubmed]
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