Disease relevance of KRT5

• A mutation in bovine keratin 5 causing epidermolysis bullosa simplex, transmitted by a mosaic sire [1].
• To test the ability of the WDSV rv-cyclin to induce cell proliferation, we have generated transgenic mice expressing the rv-cyclin in squamous epithelia from the bovine keratin-5 promoter [2].
• The purpose of the present study was to evaluate the therapeutic effect of K5 on alkali-burn-induced corneal neovascularization (NV) and to investigate its mechanism of action [3].

High impact information on KRT5

• Here, we have targeted the expression of the catalytic component of mouse telomerase, mTERT, to basal keratinocytes using the bovine keratin 5 promoter [4].
• We generated transgenic mice that overexpress PKCalpha in the basal layer of the epidermis and the outer root sheath of hair follicles under the regulation of the bovine keratin 5 promoter [5].
• With a Cre transgene under control of the bovine keratin 5 promoter we achieved 100% recombination of the Ercc1 gene in the epidermis [6].
• On the contrary, the phosphorylation levels of K5 and K14 are similar in both the soluble pool and the polymerized fraction, suggesting that phosphorylation does not play an important role in the different dynamics displayed by these two proteins [7].
• However, cultured urothelial cells synthesize a greatly increased amount of K5 and K6 keratins, which are usually expressed by stratified epithelia but present only in trace amounts in normal urothelium [8].

Biological context of KRT5

• K5 inhibits proliferation and induces apoptosis in BAECs [3].
• The effects of K5 on primary bovine aortic endothelial cells (BAECs) were determined by MTT assay, flow cytometry, transmission electron microscopy, and DNA fragmentation assay [3].
• PURPOSE: Plasminogen kringle 5 (K5) is a potent angiogenic inhibitor [3].
• The inhibitory effect of K5 on corneal NV may be by downregulation of VEGF expression [3].
• These findings identify sequences between -5.3 kb and -1.3 kb of the bovine K5 gene as being important for cell-type- and differentiation-specific gene expression both during mouse development and in the adult [9].

Anatomical context of KRT5

• K5 decreased the inflammatory index in the corneas at different time points after the alkali burn [3].

Associations of KRT5 with chemical compounds

• To produce conditional expression of genes in the mouse epidermis we have generated transgenic mouse lines in which the tetracycline-regulated transcriptional transactivators, tTA and rTA, are linked to the bovine keratin 5 promoter [10].

References