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Gene Review:

MMP20 - matrix metallopeptidase 20

Bos taurus

Li, W. et al., Den Besten, P.K. et al.

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Disease relevance of MMP20

MMP-20 cDNA was isolated from a subtracted bovine cDNA library, reconstructed into pRSET A vector, and overexpressed in BL21 Escherichia coli [1].

High impact information on MMP20

Enamelysin is a matrix metalloproteinase (MMP-20) secreted by ameloblasts, previously shown to hydrolyze recombinant amelogenin [1].
The purpose of this study was to use recombinant MMP-20 to further investigate the specific hydrolysis of peptide fragments containing cleavage sites for tyrosine-rich amelogenin peptide (TRAP) and leucine-rich amelogenin peptide (LRAP) [1].
The coding regions of bovine and porcine MMP 20 cDNAs were highly homologous and contained the same regions of predicted amino acid sequence homology with the proteinase N-terminal sequences [2].

Anatomical context of MMP20

A cDNA for bovine MMP 20 was isolated from a bovine enamel organ cDNA library using a probe generated by PCR amplification [2].

Analytical, diagnostic and therapeutic context of MMP20

The recombinant MMP-20 was purified using Mono-S ion exchange and nickel affinity chromatography [1].

References

Activation of recombinant bovine matrix metalloproteinase-20 and its hydrolysis of two amelogenin oligopeptides. Li, W., Machule, D., Gao, C., DenBesten, P.K. Eur. J. Oral Sci. (1999) [Pubmed]
Purification and sequencing of a 21 kDa and 25 kDa bovine enamel metalloproteinase. Den Besten, P.K., Punzi, J.S., Li, W. Eur. J. Oral Sci. (1998) [Pubmed]

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MMP20	Canis lupus familiaris
mmp20a	Danio rerio
MMP20	Homo sapiens
MMP20	Macaca mulatta
Mmp20	Mus musculus
MMP20	Pan troglodytes
Mmp20	Rattus norvegicus
mmp20	Xenopus (Silurana) tropicalis

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