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NEK8  -  NIMA-related kinase 8

Homo sapiens

Synonyms: JCK, NEK12A, NPHP9, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, ...
 
 
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Disease relevance of NEK8

  • Nek8 is differently expressed between normal human breast tissue and breast tumors [1].
  • Mouse myeloma cells were fused with spleen cells from BALB/c mice immunized with a melanosomal fraction separated from human melanotic melanoma cells (Mm-1-JCK) [2].
  • The aim of this work was to isolate and characterize two new mammary tumor cell lines, JCK and GCS, which were obtained from the pleural effusion and ascitic fluid, respectively, from two breast cancer patients [3].
  • RESULTS: When the effects of these agents were evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay, only MMC and DDP were effective on PAN-3-JCK, a poorly differentiated adenocarcinoma [4].
 

High impact information on NEK8

  • Nek8 activity is not cell cycle regulated, but like Nek3, levels are consistently higher in G(0)-arrested cells [5].
  • Nek8 was isolated as a beta-casein kinase activity in rabbit lung and has an N-terminal catalytic domain homologous to the Nek family of protein kinases [5].
  • Treatment of cells with nocodazole leads to dramatic reorganization of Bicd2, and correlates with Nek8 phosphorylation [5].
  • We recently established four CC cell lines, designated as SCK, JCK, Cho-CK, and Choi-CK, and report the first application of cross-species color banding (RxFISH) and multiple chromosome painting for the characterization of the chromosomal rearrangements of these CC cell lines [6].
  • Homogeneously staining regions were determined in SCK and JCK, and double minute chromosomes were found in Cho-CK [6].
 

Biological context of NEK8

  • Our data demonstrate for the first time that Nek8 is a novel tumor associated gene, and shares considerable sequence homology with the Nek family of protein kinases and may be involved in G(2)/M progression [1].
  • The open reading frame of human Nek8 encodes a 692 amino-acid protein with a calculated molecular weight of 75 kDa [1].
  • We isolated Nek8 from a primary human colon cDNA library, and found it to be highly homologous to murine Nek8 [1].
  • Up-regulation of these three proteins may be due to the nek8 mutation, and their function may be related to the signaling and structural processes in the primary cilium [7].
  • A significant increase was also found (P greater than 0.05) in cell growth and 3H-thymidine incorporation into DNA in the JCK and GCS cell lines in the presence of 17 beta estradiol at concentrations of 10(-9) and 10(-7) M, respectively, after 5 days in culture [3].
 

Anatomical context of NEK8

 

Associations of NEK8 with chemical compounds

  • MMC showed positive antitumor activity on PAN-3-JCK and PAN-12-JCK, and 5-FU was effective on PAN-12-JCK [4].
 

Analytical, diagnostic and therapeutic context of NEK8

References

  1. Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors. Bowers, A.J., Boylan, J.F. Gene (2004) [Pubmed]
  2. Melanosomal antigenic expression on the cell surface and intracellular subunits within melanogenic compartments of pigment cells: analysis by antimelanosome-associated monoclonal antibody. Hayashibe, K., Mishima, Y., Ichihashi, M., Kawai, M. J. Invest. Dermatol. (1986) [Pubmed]
  3. New cell lines of human breast cancer origin. Calaf, G., Abarca-Quinones, J., Feuilhade, F., Beaune, J., Dupre, G., Orrico, M., Barnabas-Sohi, N., Kouyoumdjian, J.C. Breast Cancer Res. Treat. (1992) [Pubmed]
  4. Chemosensitivity of human pancreatic cancer cell lines serially transplanted in nude mouse. Tomikawa, M., Kubota, T., Takahashi, S., Matsuzaki, S.W., Kitajima, M. Anticancer Res. (1998) [Pubmed]
  5. Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. Holland, P.M., Milne, A., Garka, K., Johnson, R.S., Willis, C., Sims, J.E., Rauch, C.T., Bird, T.A., Virca, G.D. J. Biol. Chem. (2002) [Pubmed]
  6. Establishment and characterization of chromosomal aberrations in human cholangiocarcinoma cell lines by cross-species color banding. Kim, D.G., Park, S.Y., You, K.R., Lee, G.B., Kim, H., Moon, W.S., Chun, Y.H., Park, S.H. Genes Chromosomes Cancer (2001) [Pubmed]
  7. Nek8 mutation causes overexpression of galectin-1, sorcin, and vimentin and accumulation of the major urinary protein in renal cysts of jck mice. Valkova, N., Yunis, R., Mak, S.K., Kang, K., Kültz, D. Mol. Cell Proteomics (2005) [Pubmed]
  8. Increased renal calcium reabsorption by parathyroid hormone-related protein is a causative factor in the development of humoral hypercalcemia of malignancy refractory to osteoclastic bone resorption inhibitors. Onuma, E., Azuma, Y., Saito, H., Tsunenari, T., Watanabe, T., Hirabayashi, M., Sato, K., Yamada-Okabe, H., Ogata, E. Clin. Cancer Res. (2005) [Pubmed]
 
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