Disease relevance of NOB1

• Functional receptors (IL1-R) for the proinflammatory cytokine interleukin 1 (IL1) were solubilized from plasma membranes of the NOB-1 subclone of murine EL4 6.1 thymoma cells using the zwitterionic detergent 3[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) [1].

High impact information on NOB1

• IL1-R was purified to apparent homogeneity from solubilized NOB-1 membranes by affinity chromatography on wheat germ agglutinin-Sepharose and IL1 alpha-Sepharose [1].
• Biological assays for interleukin 1 detection. Comparison of human T lymphocyte, murine thymocyte and NOB-1 assays [2].
• 1. The NOB1 cDNA is 1749 bp long and contains a putative open reading frame of 1239 bp [3].
• The human NOB1 gene is composed of nine exons and eight introns and is localized on human chromosome 16q22 [3].

Analytical, diagnostic and therapeutic context of NOB1

• RT-PCR analysis of mRNA from human adult tissues showed that NOB1 is expressed mainly in liver, lung and spleen [3].
• Western blot analysis showed that the molecular weight of NOB1 is about 50 KDa [3].

References