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Gene Review

YALI0E00814g  -  YALI0E00814p

Yarrowia lipolytica CLIB122

 
 
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High impact information on AOX1

  • We have identified five acyl coenzyme A (CoA) oxidase isozymes (Aox1 through Aox5) in the n-alkane-assimilating yeast Yarrowia lipolytica, encoded by the POX1 through POX5 genes [1].
  • The lipase was successfully expressed and secreted with an apparent molecular weight of 39kDa using Saccharomyces cerevisiae secretion signal peptide (alpha-factor) under the control of the methanol inducible promoter of the alcohol oxidase 1 gene (AOX1) [2].

References

  1. Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica. Wang, H.J., Le Dall, M.T., Wach, Y., Laroche, C., Belin, J.M., Gaillardin, C., Nicaud, J.M. J. Bacteriol. (1999) [Pubmed]
  2. High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Yu, M., Lange, S., Richter, S., Tan, T., Schmid, R.D. Protein Expr. Purif. (2007) [Pubmed]
 
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