Disease relevance of Hspa1b

• Hsp 72 was also detected in CA3 neurons destined to survive 7 days after ischemia [1].
• Hsp70-2 gene does not belong to the immediate early related genes that are responsible for germ cell apoptosis induced by heat stress [2].
• The results showed that the expression level of Hsp70-2 gene declined slightly at the early stage of germ cell apoptosis, and dropped dramatically when most of the germ cells were undergoing apoptosis on day 7.5 after the induction of cryptorchidism [2].

High impact information on Hspa1b

• As an example of single gene analysis, the RT1-linked heat shock-inducible heat shock genes Hsp70-1 and Hsp70-2 have been studied [3].
• Heat shock protein (Hsp)70-1 and Hsp70-2 mRNA increase greatly in most tissues, which cannot be attributed to decreased TonEBP activity [4].
• Unlike Hsp 72, Hsp 27 is constitutively expressed in the rat embryo in the absence of any thermal stress; however, the level of Hsp 27 is increased approximately 2-3-fold after thermal stress (43 degrees C, 10 min) [5].
• Hsp 72 response to a transient forebrain ischemia seemingly reflects differences in the selective ischemic vulnerability of CA1/2 and CA3 neurons [1].
• However, green tea drinking failed to prevent the vanadium-induced Hsp72 under-expression in kidney of vanadium-treated rats [6].

Anatomical context of Hspa1b

• There was no effect on the inducible cytosolic Hsp 70, Hsp 72, nor on mtHsp 70 in rho 0 cells, leading us to concluded that stress applied selectively to mitochondria elicits a specific molecular chaperone response [7].

Associations of Hspa1b with chemical compounds

• In kidney of vanadium-treated rats, Hsp73 was over-expressed by about 50% whereas Hsp72 was down-regulated by 50-80% [6].

Other interactions of Hspa1b

• The DNAs were digested with restriction enzymes BamHI, EcoRI, PstI or PvuII and assayed by probes hybridizing to all three genes or selectively to Hsp70-1, Hsp70-2 and Hsp70-3, respectively [8].
• This report suggests for the first time that Hsp70-2 gene might not inhibit the apoptosis of germ cells at the early stage in cryptorchid testes [2].

Analytical, diagnostic and therapeutic context of Hspa1b

• Hsp 72 could not be detected by Western blot analysis of proteins from embryos cultured at 40 degrees C or below [9].
• To investigate the role of Hsp70-2 gene in germ cell apoptosis induced by heat stress, its expression changes were examined in rat normal and unilateral cryptorchid testes by using in situ hybridization, immunohistochemistry, and northern blot analysis techniques [2].

References