High impact information on npy

• We report here cloning of the npy and pyy genes in zebrafish (Danio rerio) [1].
• In situ hybridization showed that neurons containing NPY mRNA have a widespread distribution in the brain, particularly in the telencephalon, optic tectum, and rhombencephalon [1].
• For analysis of some brainstem nuclei and regions, CR distribution was compared with that of complementary markers (choline acetyltransferase, glutamic acid decarboxylase, tyrosine hydroxylase, neuropeptide Y) [2].
• Here we report on a direct comparison of the pharmacological properties of these three receptors in vitro using porcine NPY with alanine substitutions in positions 33-36 as ligands and three analogues with internal deletions: [Ahx(8-20)]NPY, [Ahx(8-20), Pro(34)]NPY, and [Ahx(5-24)]NPY [3].
• We also examined the distribution of several neurotransmitter markers (tyrosine hydroxylase, neuropeptide Y, dopamine-beta-hydroxylase, and serotonin) in the olfactory bulb [4].

Biological context of npy

• This suggests that npy and pyy arose from a common ancestral gene in a chromosomal duplication event that also involved the hox gene clusters [1].
• NPY has a number of important physiological functions such as regulating food intake and reproduction [5].

Anatomical context of npy

• Functional expression of the lamprey receptor in a cell line facilitated specific binding of the three endogenous lamprey peptides NPY, peptide YY and peptide MY with picomolar affinities [6].
• Neuropeptide Y is present in some cells abutting the locus coeruleus, but these are probably not catecholamine-containing neurons [7].

Associations of npy with chemical compounds

• Cloning of a neuropeptide Y/peptide YY receptor from the Atlantic cod: the Yb receptor [8].
• Pufferfish and Zebrafish Have Five Distinct NPY Receptor Subtypes, but Have Lost Appetite Receptors Y1 and Y5 [9].
• In competition experiments the cod Yb receptor had the following rank order of potencies: porcine PYY = porcine NPY = p[Leu(31), Pro(34)]NPY > zebrafish PYY > zebrafish NPY >> NPY2-36 = NPY3-36 > NPY18-36 > bovine PP = [D-Trp(32)]NPY > BIBP3226 [10].

Analytical, diagnostic and therapeutic context of npy

• RT-PCR studies in pufferfish show that all five are expressed in the brain and may mediate NPY effects on feeding [9].

References