Disease relevance of Cp38

• The cloned genes encoding carboxylesterase E3 in the blowfly Lucilia cuprina and its orthologue in Drosophila melanogaster were expressed in Sf9 cells transfected with recombinant baculovirus [1].
• This fragment was also capable of initiating transcription of E. coli lac z RNA with the developmental profile of the native s38 gene [2].

High impact information on Cp38

• To determine if the sequences bound by the particles play a role in RNA processing, we have correlated the nascent transcript morphology of Drosophila chorion s36-1 and s38-1 genes with their nucleotide sequences [3].
• Amplification of the X-linked Drosophila chorion gene cluster requires a region upstream from the s38 chorion gene [4].
• The repetitive region was not required for developmentally regulated s38 transcription, therefore our results identified at least one element required for amplification but not for chorion gene transcription [4].
• Deletion analysis identified a 467-bp region upstream from the s38 transcription start site that contained sequences essential in cis for amplification [4].
• The otu gene was transcribed proximal to distal relative to the centromere; this was opposite to the direction of transcription of the adjacent s38 gene [5].

Biological context of Cp38

• The central domain of both proteins, which exhibits extensive sequence homology with the corresponding domains of Drosophila melanogaster proteins s36 and s38, is formed by tandem repeats of an octapeptide-X-X-X-Z-Z-Z-Z-Z- (where X = large hydrophobic residue and Z = beta-turn former residue) and its variants [6].
• Transformation experiments with an s38-1-lacZ fusion gene demonstrated that cis-regulatory sequences sufficient for the stage-specific program of s38-1 expression were confined to a 1.3 X 10(3) base-pair segment between -748 and +573 relative to the s38-1 initiation site [7].
• Here we report the molecular organization of the murine Gcm1, its spatio-temporal pattern of expression in developing placenta, and its map position at E1-E3 on murine chromosome 9 [8].
• The Cbl family of proteins act as E3 ubiquitin-protein ligases and have been associated with the down regulation of a variety of receptor tyrosine kinases [9].
• No change in the amino acid sequences was found in Ace1, while two amino acid substitutions, Ser431Phe and Ala302Ser, were detected between resistant and susceptible strains in Ace2 [10].

Associations of Cp38 with chemical compounds

• EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue [11].
• Two acetylcholinesterase (AChE) genes, Ace1 and Ace2, have been cloned from cotton aphid, Aphis gossypii Glover, using the rapid amplification of cDNA ends (RACE) technique [12].

Other interactions of Cp38

• P element-mediated gene transfer was used to study the regulation of genes encoding the major chorion proteins s15-1 and s38-1 [7].

References