High impact information on RpS13

• In vivo assays using short-lived proteasome substrates confirmed that the 26S ubiquitin-dependent degradation pathway is compromised in S13-depleted cells [1].
• Use of RNA interference and complementation to study the function of the Drosophila and human 26S proteasome subunit S13 [1].
• The S13 subunit may possess multiple functions, including a deubiquitinylating activity and distinct activities essential for cell cycle progression that require the conserved C120 residue [1].
• To address the functional role of S13, we combined double-stranded RNA interference (RNAi) against the Drosophila proteasome subunit DmS13 with expression of wild-type and mutant forms of the homologous human gene, HS13 [1].
• Loss of the S13 subunit in metazoan cells leads to increased levels of ubiquitin conjugates, cell cycle defects, DNA overreplication, and apoptosis [1].

Biological context of RpS13

• Here, we report a previously undescribed Minute locus that maps to 32A on chromosome 2L; this Minute allele is named P¿lacW¿M(2)32A1 and the gene M(2)32A [2].

Other interactions of RpS13

• A novel Drosophila Minute locus encodes ribosomal protein S13 [2].

Analytical, diagnostic and therapeutic context of RpS13

• Sequence analyses revealed that it encodes the Drosophila homologue of eukaryotic ribosomal protein S13 [2].

References