High impact information on porin

• 1) The CG17137 gene may express a porin with a functional role in D. melanogaster [1].
• Genome sequencing of the fruit fly Drosophila melanogaster revealed the presence of three additional porin-like genes [1].
• Sometimes channels exhibited closure and voltage gating, but for CG17140 this occurred at much higher voltages than is typical for VDAC [2].
• By in situ hybridization, we have localized agporin at region 35D on the right arm of A. gambiae chromosome 3, which is homologous to the 2L chromosomal arm of D. melanogaster where the porin gene resides [3].
• It was found that this D. melanogaster porin gene contains five exons, numbered IA (115 bp), IB (123 bp), II (320 bp), III (228 bp) and IV (752 bp) [4].

Biological context of porin

• The D. melanogaster porin cDNA was used as a probe for in situ hybridization to polytenic salivar gland chromosomes [5].
• A subclone of 1T1, containing the porin-like sequence, was thus used as a probe for re-screening a cDNA library and several positive clones were plaque-purified [5].
• Thus, also in Drosophila melanogaster porin polypeptide(s) belong(s) to a multigene family [5].
• Sequence and expression pattern of the Drosophila melanogaster mitochondrial porin gene: evidence of a conserved protein domain between fly and mouse [4].
• While porin, CG17137, and CG31722-B are expressed to different degrees during embryogenesis, all of these proteins are dramatically reduced relative to cytochrome c content during larvogenesis [6].

Anatomical context of porin

• Expression and localization in spermatozoa of the mitochondrial porin isoform 2 in Drosophila melanogaster [7].
• The voltage-dependent anion channel (VDAC, also known as porin) is an abundant protein in the outer mitochondrial membrane that forms transmembrane channels permeable to solutes [8].
• Further expression analysis showed that CG17137 and CG31722-B are abundant in testes, while porin is ubiquitously expressed [6].
• The porin from adults' mitochondria was fully characterized [9].
• The recombinant DmPorin2 protein is able to form channels similar to those formed by DmPorin1 reconstituted in artificial membranes [1].

Other interactions of porin

• Functional characterization of a second porin isoform in Drosophila melanogaster. DmPorin2 forms voltage-independent cation-selective pores [1].

Analytical, diagnostic and therapeutic context of porin

• Northern blots demonstrated that the porin gene is a housekeeping one: three messages of approx. 1.2-1.6 kbp are transcribed in every fly developmental stage that was studied [4].

References