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Gene Review:

Tb927.2.6000 - glycosyl transferase

Trypanosoma brucei brucei TREU927

Redpath, M.B. et al., Subramanya, S. et al., Gruszynski, A.E. et al., Fournier, D. et al., Armah, D.A. et al., et al.

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Disease relevance of Tb927.2.6000

The sensitivity of acetylcholinesterases (AChEs) from Musca domestica and from Drosophila melanogaster to the phosphatidylinositol-specific phospholipase C from Bacillus cereus and to the glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei was investigated [1].
Glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC) is expressed in Trypanosoma brucei, a protozoan parasite that causes human African trypanosomiasis [2].

High impact information on Tb927.2.6000

Surface biotinylation assays indicate that GPI-PLC does gain access to extracellular VSG, suggesting that this mode is primed in the starting short stumpy population [3].
However, GPI hydrolysis has been considered unimportant because (i) GPI-PLC null mutants are fully viable and (ii) cytosolic GPI-PLC is localized away from cell surface VSG [3].
A role for the dynamic acylation of a cluster of cysteine residues in regulating the activity of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei [4].
In vivo cross-linking revealed the oligomeric state of GPI-PLC during latency and after enzyme activation [5].
Homotetramers dominated in Nonidet P-40, and dimers and monomers of GPI-PLC were the major species in 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate [5].

Biological context of Tb927.2.6000

Together, these observations place Cys80 and Gln81 at, or close to, the active site of GPtdIns-PLC from T. brucei [6].
However, an effective response was restored upon reintegration of the GPI-PLC gene in the genome (PLC+) [7].
The relegation of short stumpy surface GPI-PLC to a secondary role in differentiation suggests that it may play a more important role as a virulence factor within the mammalian host [8].
Restriction enzyme fragments located to the 3' side of the T. cruzi hsp100 gene were then sequenced and found to contain a gene that encodes a polypeptide (TcPLC1) that has 46% amino acid sequence identity with the T. brucei GPI-PLC including most of the key residues involved in inositol binding and the catalytic histidine [9].
Conservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzi [9].

Anatomical context of Tb927.2.6000

A catalytically inactive Gln81Leu mutant of GPI-PLC failed to cleave GPIs despite being transported from glycosomes to the ER after hypo-osmotic or mild alkaline treatment of the parasites [2].

Associations of Tb927.2.6000 with chemical compounds

Sulfhydryl reagents inactivate GPtdIns-PLC, suggesting that a Cys could be close to the enzyme active site [6].

References

Acetylcholinesterases from Musca domestica and Drosophila melanogaster brain are linked to membranes by a glycophospholipid anchor sensitive to an endogenous phospholipase. Fournier, D., Bergé, J.B., Cardoso de Almeida, M.L., Bordier, C. J. Neurochem. (1988) [Pubmed]
Regulated cleavage of intracellular glycosylphosphatidylinositol in a trypanosome. Subramanya, S., Mensa-Wilmot, K. FEBS J. (2006) [Pubmed]
Surface coat remodeling during differentiation of Trypanosoma brucei. Gruszynski, A.E., DeMaster, A., Hooper, N.M., Bangs, J.D. J. Biol. Chem. (2003) [Pubmed]
A role for the dynamic acylation of a cluster of cysteine residues in regulating the activity of the glycosylphosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Paturiaux-Hanocq, F., Hanocq-Quertier, J., de Almeida, M.L., Nolan, D.P., Pays, A., Vanhamme, L., Van den Abbeele, J., Wasunna, C.L., Carrington, M., Pays, E. J. Biol. Chem. (2000) [Pubmed]
Tetramerization of glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei. Armah, D.A., Mensa-Wilmot, K. J. Biol. Chem. (2000) [Pubmed]
Roles of Gln81 and Cys80 in catalysis by glycosylphosphatidylinositol-phospholipase C from Trypanosoma brucei. Rashid, M.B., Russell, M., Mensa-Wilmot, K. Eur. J. Biochem. (1999) [Pubmed]
Mild acid stress as a differentiation trigger in Trypanosoma brucei. Rolin, S., Hancocq-Quertier, J., Paturiaux-Hanocq, F., Nolan, D.P., Pays, E. Mol. Biochem. Parasitol. (1998) [Pubmed]
Regulation of surface coat exchange by differentiating African trypanosomes. Gruszynski, A.E., van Deursen, F.J., Albareda, M.C., Best, A., Chaudhary, K., Cliffe, L.J., Del Rio, L., Dunn, J.D., Ellis, L., Evans, K.J., Figueiredo, J.M., Malmquist, N.A., Omosun, Y., Palenchar, J.B., Prickett, S., Punkosdy, G.A., van Dooren, G., Wang, Q., Menon, A.K., Matthews, K.R., Bangs, J.D. Mol. Biochem. Parasitol. (2006) [Pubmed]
Conservation of genetic linkage between heat shock protein 100 and glycosylphosphatidylinositol-specific phospholipase C in Trypanosoma brucei and Trypanosoma cruzi. Redpath, M.B., Carnall, N., Webb, H., Courel, M., Amorim, A., Güther, M.L., Cardoso de Almeida, M.L., Carrington, M. Mol. Biochem. Parasitol. (1998) [Pubmed]

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