Disease relevance of RPMS1

• Reverse transcription-PCR analysis revealed that BART transcripts with the splicing pattern that generates the RPMS1 open reading frame are commonly expressed in EBV-positive lymphoblastoid cell lines and are also detected in Hodgkin's disease tissues [1].

High impact information on RPMS1

• Our results demonstrated that RNAs with splicing diagnostic for transcripts containing the BART RPMS1 and BARFO open-reading frames (ORFs) were expressed in CD19(+) but not in CD23(+) B cells isolated from peripheral blood of healthy individuals [2].
• The effect of RPMS on NotchIC function was further examined in a muscle cell differentiation assay where RPMS was found to partially reverse NotchIC-mediated inhibition of differentiation [1].
• The BARTs detected were shown possibly to encode the RPMS1 and BARF0 proteins, based on their splicing [3].

Analytical, diagnostic and therapeutic context of RPMS1

• The mechanism of RPMS action was examined in cotransfection and mammalian two-hybrid assays [1].
• Consistent with this function, immunofluorescence assays performed on cotransfected cells showed that there was colocalization of RPMS with NotchIC and with EBNA2 in intranuclear punctate speckles [1].

References