Disease relevance of aroA

• Attenuation and persistence of and ability to induce protective immunity to a Staphylococcus aureus aroA mutant in mice [1].
• Intramammary immunization with the aroA mutant stimulated both Th1 and Th2 responses in the mammary gland, as ascertained by reverse transcription-PCR, and induced significant protection from challenge with either the parental wild-type or a heterologous strain isolated from a cow with mastitis [1].

High impact information on aroA

• Complementation of the aroA mutant with a plasmid carrying aroA recovered the wild-type phenotype [1].
• The Staphylococcus aureus aroA gene, which encodes 5-enolpyruvylshikimate-3-phosphate synthase, was used as a target for the amplification of a 1,153-bp DNA fragment by PCR with a pair of primers of 24 and 19 nucleotides [2].
• Detection of enterotoxin genes seg, seh, sei, and sej and of a novel aroA genotype in Jordanian clinical isolates of Staphylococcus aureus [3].
• Genotyping by PCR-restriction fragment length polymorphism analysis of the aroA gene revealed that 39% of the isolates were type A and 11% were type B, and 50% displayed a novel (N) genotype [3].
• An aroA insertion mutant isolated by allelic replacement was employed in genetic mapping experiments which demonstrated the gene order thy aroA tyrB in SmaI fragment A of the S. aureus 8325-4 chromosome [4].

Biological context of aroA

• A revised consensus sequence for the aroA gene product EPSP synthase binding site for its substrate (phosphoenolpyruvate) and an inhibitor (glyphosate) is proposed [4].

Anatomical context of aroA

• The aroA mutant showed decreased ability to persist in the lungs, spleens, and mammary glands of mice [1].

References