High impact information on NR5A1

• These results add alpha2M to the list of nonsteroidal genes regulated by SF-1 in the gonads and provide the first evidence that COUP-TF has a specific role in regulating ovarian gene activity [1].
• When site-specific mutations were made in either the SF-1 -binding site or the two COUP-TF direct repeat (DR1 and DR2) binding sites in the context of the intact promoter, specific changes in the functional activity of this novel region of the alpha2M promoter were observed [1].
• Other DNA sequences, not bound by SF-1, are also involved in transcriptional regulation of the rat P450c17 gene in both cell types [2].
• We show that during embryonic and postnatal development, AMH, SF1 and SOX9 are expressed in the epithelium of testicular-like cords and in the thickened part but not in the flattened part of the epithelium of composite lacunae [3].
• The WT1 gene was highly expressed in both sexes, whereas SF1 expression was clearly higher in developing ovaries compared to testes [4].

Biological context of NR5A1

• In the proximal promoter sequence of sb-AMH, a steroidogenic factor-1 (SF-1) binding site was present; however other regulatory sequences essential for transcriptional activation of AMH in mammals were absent [5].
• In addition, we also cloned the 5'-untranslated region (4.6kb) of the SF-1/Ad4BP gene with exons I and II [6].
• The E and CCAAT boxes moderately affected promoter activity and the GA-rich element and TATA box were essential for the expression of the chicken SF-1/Ad4BP gene [7].
• It encoded a protein of 468 amino acids, and its open reading frame (ORF) shared 70% similarity with that of chicken OR2.1 (a SF-1/Ad4BP homologue) and 62% with bovine SF-1/Ad4BP [6].
• Methylation interference experiments show the contact points and, by electrophoretic retardation analysis with SF1 and SF1-mutated oligonucleotides, the critical role of these contacts points on the formation of the DNA-protein complex could be demonstrated [8].

Anatomical context of NR5A1

• Moreover, SF-1/Ad4BP plays a critical role in the sexual differentiation of gonads in mammals [9].
• OR2.1 consists of 3255 bp, is expressed in the adrenal glands and gonads, and is considered to be the chicken counterpart of mammalian SF-1/Ad4BP [9].
• SF-1/Ad4BP mRNA was expressed in the testes, brains, adrenals/kidneys and spleens, but not ovaries, of adult frogs [6].

Associations of NR5A1 with chemical compounds

• 2. IOB at high concentrations provoked fragmentation of SF1 at its tryptophan residues [10].

Analytical, diagnostic and therapeutic context of NR5A1

• Molecular cloning and expression of the SF-1/Ad4BP gene in the frog, Rana rugosa [6].
• Using a quantitative Western blot analysis system, we documented changes in SF-1 protein in the gonads of developing tadpoles [11].

References