Disease relevance of DBN1

• Those on motoneurons codistribute with the actin-associated component drebrin, as do the clusters on ciliary ganglion neurons, but the clusters on preganglionic neurons do not [1].

High impact information on DBN1

• The difference in the time course of expression and tissue distribution of each drebrin suggests that the machinery of alternative splicing site selection of the drebrin gene is regulated in a developmental stage-dependent and tissue-specific manner [2].
• Previously we isolated two cDNAs corresponding to the embryonic drebrin mRNAs from a chick embryo cDNA library [2].
• Cloning and sequencing of the drebrin gene revealed that ins1 and ins2 were independently encoded by separate exons and three drebrin isoforms were thought to arise by alternative splicing from a single drebrin gene [2].
• Nucleotide sequences of two embryonic drebrins, developmentally regulated brain proteins, and developmental change in their mRNAs [3].
• Southern blot analysis showed that the chicken genome has a single copy of the drebrin gene per haploid complement [3].

Biological context of DBN1

• These findings suggest that the multiple forms of drebrins result from alternative splicing of the single drebrin gene during neural development [3].

Anatomical context of DBN1

• These results indicate that the expression of drebrin in kitten visual cortex is restricted to the early postnatal period and suggest that it may play an important role in the experience-dependent modification of cortical circuitry during the sensitive period [4].
• A monoclonal antibody (MAb M2F6) raised against drebrin, was found to label the neuropil of the kitten visual cortex in the early postnatal period [4].

Analytical, diagnostic and therapeutic context of DBN1

• Molecular cloning of a developmentally regulated brain protein, chicken drebrin A and its expression by alternative splicing of the drebrin gene [2].
• The expression of and developmental changes in drebrin were studied in cat visual cortex using immunohistochemistry and immunoblot analysis [4].
• Upon immunoblot analysis after SDS-PAGE of protein from the kitten visual cortex, MAb M2F6 was found to recognize two protein bands with molecular weights of 130 kDa (drebrin E) and 140 kDa (drebrin A) [4].

References