High impact information on GLTP

• These results suggest that the glycolipid transfer protein from pig brain has specificity to hydroxyl groups present in the sugar residue directly linked to either ceramide or diacylglycerol [1].
• Net mass transfer of galactosylceramide facilitated by glycolipid transfer protein from pig brain: a monolayer study [2].
• No significant decrease was found in the binding affinity of NEM-treated GLTP to pyrene-labeled galactosylceramide (PyrGalCer) [3].
• Treatment of GLTP with 1.27 mM HgCl2 resulted in a formation of components unresolvable on SDS-PAGE and also resulted in a reduction of the transfer activity to one-third [4].
• Treatment with 2-mercaptoethanol restored the activity of GLTP inactivated by HgCl2, whereas the activity inactivated by CuSO4 was not restored by treatment with 2-mercaptoethanol [4].

Associations of GLTP with chemical compounds

• However, no obvious change in the binding affinity of GLTP to PyrGalCer was observed by HgCl2 treatment [4].
• The sulfhydryl group content of GLTP was estimated by the use of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) [4].
• Oxidation of GLTP catalyzed by CuSO4 resulted in a decrease in the transfer activity and an increase in the apparent binding affinity of GLTP to 1-O-(beta-D-galactopyranosyl)-N-[10-(1-pyrenyl)decanoyl]-D-erythro- sphingosine (PyrGalCer) [4].
• (Correa-Freire, M.C., Barenholz, Y. and Thompson, T.E. (1982) Biochemistry 21, 1244-1248) to measure glucosylceramide transfer between phospholipid bilayers has been applied to the determination of the activity of glycolipid transfer protein purified from pig brain [5].

Analytical, diagnostic and therapeutic context of GLTP

• Purified GLTP migrates as two bands of different mobility in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions [4].
• By circular dichroism measurements, it was found that a small decrease in the magnitude of mean residue ellipticity but no significant change in ellipticity spectrum was brought about either upon the modification of GLTP by Na2S4O6 or upon the formation of an intramolecular disulfide bond in GLTP [3].

References