Disease relevance of FN1

• Hepatoma cells cultured as monolayers on fibronectin-coated surfaces served as targets for immune splenocytes in a 3H release cytotoxicity assay in vitro [1].
• The same level of activity is observed when these synthetic peptides were assayed for inhibition of Fn binding to cells of Staphylococcus aureus [2].
• Fibronectin is the major fibroblast chemoattractant in rabbit anti-glomerular basement membrane disease [3].
• Antibodies to fibronectin and creatine kinase are considered autoantibodies, and the underlying mechanism responsible for their production in syphilis is discussed [4].
• Modulation of expression of delayed hypersensitivity by mycobacterial antigen 85 fibronectin-binding proteins [5].

High impact information on FN1

• Monocyte receptors for fibronectin characterized by a monoclonal antibody that interferes with receptor activity [6].
• Thus, at least functionally, the antibody appears to recognize the plasma membrane receptor for fibronectin [6].
• Clusterin and fibronectin promoted cell adhesion to the same extent [7].
• We documented increased donor coronary artery smooth muscle cell fibronectin protein synthesis and mRNA compared to host [8].
• Upregulation of fibronectin synthesis by interleukin-1 beta in coronary artery smooth muscle cells is associated with the development of the post-cardiac transplant arteriopathy in piglets [8].

Chemical compound and disease context of FN1

• Induction of fibronectin-binding proteins and increased adhesion of quinolone-resistant Staphylococcus aureus by subinhibitory levels of ciprofloxacin [9].
• Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfaces [10].
• Successful EDJ mapping was carried out in normal pig skin (NPS), ethanol-treated IPPSFs, and HD-treated IPPSFs using the following antibodies: laminin, type IV collagen, fibronectin, GB3 (Nicein), bullous pemphigoid (BP), and epidermolysis bullosa acquisita (EBA) [11].
• NPC 15669 treatment was associated with diminished endothelin-1, TxB2 levels and increased fibronectin, 6-keto-PGF1a, Protein C and total Protein S concentrations in the setting of preconditioned myocardial infarction [12].
• Comparison of triphenyltetrazolium chloride (TTC) staining versus detection of fibronectin in experimental myocardial infarction [13].

Biological context of FN1

• Localization of a cryptic binding site for tenascin on fibronectin [14].
• Fibronectin and tenascin are large extracellular matrix proteins that interact with each other and with integrin receptors to regulate cell growth and movement [14].
• The results demonstrate that fibronectin promotes epithelial migration in the cornea and thus plays an important role in corneal wound healing [15].
• Our studies strongly suggest that the fibronexus is an important in vivo cell surface adhesion site functioning in wound repair, and perhaps within fibronectin-rich tissues during embryogenesis, tumor growth, and inflammation [16].
• Conversely, when fibroblasts were in fibronectin-rich cultures, PDGF-BB promoted alpha 3 and alpha 5, but not alpha 2, mRNA stability [17].

Anatomical context of FN1

• A provisional stromal matrix of cross-linked fibrin and fibronectin was initially deposited in both tumors and wounds and was subsequently replaced by granulation tissue containing collagen types I and III [18].
• Fibronectin mediates the attachment of fibroblasts but not of epidermal cells to collagen [19].
• Megakaryocytes spread with an active ruffled membrane in response to ADP (1-100 microM), thrombin (1.0 U/ml), and arachidonic acid (50 microM) but responded to collagen surfaces only if fibronectin was added to the cultures [20].
• Fibronectin-containing microfibrils link fibroblasts to each other and to collagen bundles and thereby provide transduction cables across the wound for contraction [21].
• Endothelial cell fibronectin synthesis was determined after radiolabeling with [35S]-methionine in serum-free medium, gelatin-sepharose extraction of the culture medium and resolution on 5% SDS-PAGE [22].

Associations of FN1 with chemical compounds

• Using a heparin-Sepharose column, we localize the second heparin binding site to the fifth fibronectin type III domain [23].
• [35S]Methionine endogeneously labeled fibronectin was immunoprecipitated from supernatants and cellular lysates and displayed on sodium dodecyl sulfate polyacrylamide slab gel electrophoresis [24].
• Secondary cells grown in CS alone required laminin or Type IV collagen, whereas cells grown in CS plus FGF required fibronectin or heparan sulfate [25].
• These results indicate that GGP is a glycoprotein which is distinct from fibronectin [26].
• The protein was bound strongly to the arginine column and thus could be separated from fibronectin [26].

Physical interactions of FN1

• Tenascin-C binds heparin by its fibronectin type III domain five [23].

Regulatory relationships of FN1

• Coronary artery endothelial interleukin-1 beta mediates enhanced fibronectin production related to post-cardiac transplant arteriopathy in piglets [22].
• Likewise, IL-1 beta-stimulated fibronectin synthesis was downregulated to control levels when TNF-alpha neutralizing antibodies were added [27].
• The 45 kDa collagen-binding fragment of fibronectin induces matrix metalloproteinase-13 synthesis by chondrocytes and aggrecan degradation by aggrecanases [28].

Other interactions of FN1

• To investigate the mechanism of action of p38 MAPK, we explored its cellular localization and that of its indirect substrate, the heat shock protein Hsp27, during SMC spreading on fibronectin in the presence and absence of PDGF [29].
• In contrast, mRNA levels coding for type I collagen, fibronectin and GAPDH (used as control of cellular activity) were not modified [30].
• Therefore, basic protein BHB-2 belongs to a new family of DQH sperm surface proteins that are homologous to the acidic proteins from bull and stallion seminal plasma, to the collagen binding domain II in fibronectin and to the leucocyte cell-cell adhesion regulator, but are not homologous to AQN or AWN spermadhesins [31].
• Immunohistochemical staining to desmosomal proteins, cellular fibronectin, laminin 1, laminin 5, collagen IV, collagen VII, p53, Bcl-2, and PCNA was performed on weanling pig skin exposed to vesicating doses of HD, GB3, an antibody to laminin 5, showed a progressive decrease with loss of expression during the time period of clinical vesiculation [32].
• In lavage, only SP-D, alpha (2)-macroglobulin and fibronectin were detected [33].

Analytical, diagnostic and therapeutic context of FN1

• In this study we use surface plasmon resonance to examine the interaction between various fragments of fibronectin and tenascin to further characterize and localize the binding sites [14].
• We previously described in piglets after heterotopic cardiac transplantation the early development of a coronary arteriopathy characterized by increased immunostaining for fibronectin and interleukin-1 beta (IL-1 beta) in the vessel wall [8].
• At 7-9 d, most of the actin fibers were observed to be coincident with congruent fibronectin fibers using double-label immunofluorescence microscopy [16].
• This conspicuous co-distribution of fibronectin and actin fibers prompted us to look for fibronexuses at the myofibroblast surface with electron microscopy [16].
• Using immunoelectron microscopy on ultrathin cryosections, we confirmed that the densely staining external portion of these various FNXs does indeed contain fibronectin [16].

References