The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

trpE  -  anthranilate synthase component I

Escherichia coli UTI89

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of trpE

 

High impact information on trpE

  • Upon induction of trpE with indoleacrylic acid, large amounts of trpE-v-rel fusion proteins were produced by the bacteria carrying these recombinant plasmids [6].
  • Portions of the v-rel gene were joined to the 5' segment of the trpE gene [6].
  • The hybrid protein encoded by the trpE/CBP6 fusion has been purified from E. coli and used as an antigen for antibody production [7].
  • The ASA2 cDNA complemented a trpE nonsense mutant Escherichia coli strain, allowing growth on 300 microm 5MT-containing minimal medium without tryptophan, and cell extracts contained feedback-insensitive AS activity [8].
  • In studies with a trpE promoter-strength measuring system we observed that constructs containing the Escherichia coli trp promoter and its adjacent transcribed region yielded lower levels of trpE protein than were expected [9].
 

Chemical compound and disease context of trpE

 

Biological context of trpE

  • These findings are interpreted in terms of alternative models in which the primary effect of mutational changes that elevate trpE expression is to increase trpE mRNA translation, versus increasing trpE mRNA stability [9].
  • Sequence changes preceding a Shine-Dalgarno region influence trpE mRNA translation and decay [9].
  • Here, we have expressed the open reading frame as trpE fusion proteins and used them to generate antibodies [14].
  • When the trp operon of Escherichia coli contains either of two deletions that fuse the initial portion of the leader region to the distal segments of the trpE gene, novel fusion polypeptides are produced [15].
  • Nucleotide sequences upstream of trpE and trpG encode putative leader peptides similar in sequence to leader peptides found in other bacterial species, and the phenotypes of the mutants strongly suggest that transcription of these operons is regulated solely by attenuation [16].
 

Anatomical context of trpE

 

Associations of trpE with chemical compounds

  • A plasmid directing the synthesis of a G-protein-like protein (containing the NH2-terminal segment of seven amino acids encoded by the trpE gene fused to the complete G-protein sequence lacking only its NH2-terminal methionine) could be transformed into trpR+ (repressed) but not into trpR- (derepressed) cells [18].
  • Analysis of attenuation by measurement of trp mRNA synthesis was facilitated by constructing a plasmid (pAD1) containing the rpoC transcription terminator inserted early in trpE [19].
  • An analysis of deoxyribonucleic acid molecules carrying deletions ending within the trp regulatory elements and a deoxyribonucleic acid molecule carrying a deletion within trpE indicates that a fragment of 8.3 X 10(5) daltons contains at least part of the trp promoter, the entire trp leader region, and part of the trpE gene [20].
  • In bacteria, the first reaction of the tryptophan biosynthetic pathway involves the conversion of chorismate and glutamine to anthranilate by the action of anthranilate synthase, which is composed of the alpha (trpE gene product) and beta (trpG gene product) subunits [13].
  • In addition, cDNA fragments containing different gp80 coding regions were used to construct trpE/gp80 gene fusions in the expression vector pATH10 [21].
 

Analytical, diagnostic and therapeutic context of trpE

References

  1. Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of 3-methylcrotonoyl-CoA carboxylase: identification of the biotin carboxylase and biotin-carrier domains. Song, J., Wurtele, E.S., Nikolau, B.J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  2. Cloning and expression of a membrane antigen of Entamoeba histolytica possessing multiple tandem repeats. Stanley, S.L., Becker, A., Kunz-Jenkins, C., Foster, L., Li, E. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  3. Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues. Freistadt, M.S., Kaplan, G., Racaniello, V.R. Mol. Cell. Biol. (1990) [Pubmed]
  4. Nucleotide sequence of the Corynebacterium glutamicum trpE gene. Heery, D.M., Dunican, L.K. Nucleic Acids Res. (1990) [Pubmed]
  5. Nucleotide sequence of trpE of Salmonella typhimurium and its homology with the corresponding sequence of Escherichia coli. Yanofsky, C., vanCleemput, M. J. Mol. Biol. (1982) [Pubmed]
  6. Expression of the oncogene of avian reticuloendotheliosis virus in Escherichia coli and identification of the transforming protein in reticuloendotheliosis virus T-transformed cells. Herzog, N.K., Bose, H.R. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  7. Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b. Dieckmann, C.L., Tzagoloff, A. J. Biol. Chem. (1985) [Pubmed]
  8. Tissue culture-specific expression of a naturally occurring tobacco feedback-insensitive anthranilate synthase. Song, H.S., Brotherton, J.E., Gonzales, R.A., Widholm, J.M. Plant Physiol. (1998) [Pubmed]
  9. Sequence changes preceding a Shine-Dalgarno region influence trpE mRNA translation and decay. Cho, K.O., Yanofsky, C. J. Mol. Biol. (1988) [Pubmed]
  10. Fusion proteins containing androgen receptor sequences and their use in the production of poly- and monoclonal anti-androgen receptor antibodies. Chang, C.S., Whelan, C.T., Popovich, T.C., Kokontis, J., Liao, S.T. Endocrinology (1989) [Pubmed]
  11. Complementation of a trpE deletion in Escherichia coli by Spirochaeta aurantia DNA encoding anthranilate synthetase component I activity. Brahamsha, B., Greenberg, E.P. J. Bacteriol. (1987) [Pubmed]
  12. Acetylcholine interactions with tryptophan-184 of the alpha-subunit of the nicotinic acetylcholine receptor revealed by transferred nuclear Overhauser effect. Fraenkel, Y., Gershoni, J.M., Navon, G. FEBS Lett. (1991) [Pubmed]
  13. Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri. Bauby, H., Saint Girons, I., Picardeau, M. Microbiology (Reading, Engl.) (2003) [Pubmed]
  14. Expression and identification of p90 as the murine mitochondrial glycerol-3-phosphate acyltransferase. Yet, S.F., Lee, S., Hahm, Y.T., Sul, H.S. Biochemistry (1993) [Pubmed]
  15. Translation of the leader region of the Escherichia coli tryptophan operon. Miozzari, G.F., Yanofsky, C. J. Bacteriol. (1978) [Pubmed]
  16. Effects of mutations in the Pseudomonas putida miaA gene: regulation of the trpE and trpGDC operons in P. putida by attenuation. Olekhnovich, I., Gussin, G.N. J. Bacteriol. (2001) [Pubmed]
  17. Overexpression and simple purification of human immunodeficiency virus-1 gag epitope derived from a recombinant antigen in E. coli and its use in ELISA. Sohn, M.J., Chong, Y.H., Chang, J.E., Lee, Y.I. J. Biotechnol. (1994) [Pubmed]
  18. Conditional expression of the vesicular stomatitis virus glycoprotein gene in Escherichia coli. Rose, J.K., Shafferman, A. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  19. Regulation of tryptophan operon expression by attenuation in cell-free extracts of Escherichia coli. Das, A., Crawford, I.P., Yanofsky, C. J. Biol. Chem. (1982) [Pubmed]
  20. Tryptophan-transducing bacteriophages: in vitro studies with restriction endonucleases HindII + III and Escherichia coli ribonucleic acid polymerase. Jones, B.B., Reznikoff, W.S. J. Bacteriol. (1977) [Pubmed]
  21. Mapping of the monoclonal antibody 80L5C4 epitope on the cell adhesion molecule gp80 of Dictyostelium discoideum. Kamboj, R.K., Siu, C.H. Biochim. Biophys. Acta (1988) [Pubmed]
  22. Molecular cloning and the nucleotide sequence of the Clostridium thermocellum trpE gene. Sato, S., Nakada, Y., Hon-nami, K., Yasui, K., Shiratsuchi, A. J. Biochem. (1989) [Pubmed]
  23. Mapping of the immunoreactive domains of a small nuclear ribonucleoprotein-associated Sm-D autoantigen. Rokeach, L.A., Jannatipour, M., Haselby, J.A., Hoch, S.O. Clin. Immunol. Immunopathol. (1992) [Pubmed]
 
WikiGenes - Universities