High impact information on TpnC73F

• Similar, but not completely equivalent, TpnC gene repertoires have been identified in the Anopheles gambiae and Apis mellifera genomes [1].
• The scallop TnC gene is constructed from five exons and four introns, and positions of introns are identical with chordate TnC genes, although the scallop TnC possesses no corresponding intron to the fourth intron of chordates [2].
• The TnC gene is a single copy gene in the scallop, thus they are expressed through the alternative splicing from the same gene [2].
• Finally, the other two genes, DmTpnC47D and DmTpnC73F, show a high embryonic/larval expression and in adults are expressed almost exclusively in the abdomens [3].
• The third gene, located within the 41C subdivision of chromosome 2, encodes a more distantly related troponin-C isoform that accumulates only within adults [4].

Biological context of TpnC73F

• This suggests that the common ancestor gene of the TnC superfamily might also be a five exons-four introns structure [2].

Analytical, diagnostic and therapeutic context of TpnC73F

• Using low-stringency hybridization and polymerase chain reaction (PCR)-based DNA amplification, we have isolated three Drosophila melanogaster genes that encode troponin-C isoforms and one specifying a protein that is closely related to calmodulin [4].
• Here we report the generation of a FRET-based calcium biosensor employing troponin C as calcium-binding moiety that is fast, is stable in imaging experiments, and shows a significantly enhanced fluorescence change [5].

References